Use of polymerase chain reaction and restriction enzyme analysis to directly detect and identify Salmonella typhimurium in food

被引:37
作者
Cocolin, L
Manzano, M
Cantoni, C
Comi, G
机构
[1] Univ Udine, Dipartimento Sci Alimenti, Fac Agr, I-33100 Udine, Italy
[2] Univ Milan, Ist Ispezione Alimenti Origin Anim, Fac Med Vet, Milan, Italy
关键词
D O I
10.1111/j.1365-2672.1998.00575.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A primer set of oligonucleotides (Salm 3 and Salm 4) from the invA gene of Salmonellae has been evaluated for the specfic detection of Salmonella spp. by the polymerase chan reaction (PCR). This primer set amplified 33 Salmonella serovars but did not amplify 16 non-Salmonella bacteria. Moreover, after PCR amplification, it was possible to identify Salm. typhimurium by restriction enzyme analysis. The PCR-RE method developed could represent a helpful tool for detecting Salmonella spp., and for directly and rapidly identifying Salm. typhimurium in food.
引用
收藏
页码:673 / 677
页数:5
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