Synthesis of protein mimics with nonlinear backbone topology by a combined recombinant, enzymatic, and chemical synthesis strategy

被引：29

作者：

Pritz, Stephan ^{[1
]}

Kraetke, Oliver ^{[1
]}

Klose, Annerose ^{[1
]}

Klose, Jana ^{[1
]}

Rothemund, Sven ^{[1
]}

Fechner, Klaus ^{[1
]}

Bienert, Michael ^{[1
]}

Beyermann, Michael ^{[1
]}

机构：

[1] Leibniz Inst Mol Pharmacol, Abt Peptidchemie & Biochem, D-13125 Berlin, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 19期

关键词：

chemical ligation; enzymatic ligation; protein design; receptor mimics;

D O I：

10.1002/anie.200705718

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) The synthesis of a 23-kDa protein that mimics the ligand-binding extracellular part of a G-protein-coupled receptor shows the potential of a combined recombinant, enzymatic, and chemical synthesis (CRECS) strategy. The mimic of the corticotropin-releasing factor receptor, synthesized from single domains by chemical ligation and sortase A-mediated coupling, has a high affinity for natural ligands. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3642 / 3645

页数：4

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