Differential kinetics of [I-123]beta-CIT binding to dopamine and serotonin transporters

被引:28
作者
Fujita, M
Takatoku, K
Matoba, Y
Nishiura, M
Kobayashi, K
Inoue, O
Nishimura, T
机构
[1] OSAKA UNIV,SCH MED,BIOMED RES CTR,DIV TRACER KINET,OSAKA 565,JAPAN
[2] DAIICHI RADIOISOTOPE LABS LTD,CHIBA 28915,JAPAN
[3] NIPPON MED COLL,DEPT PHYSIOL,TOKYO 113,JAPAN
[4] OSAKA UNIV,FAC MED,SCH ALLIED HLTH SCI,DEPT MED PHYS,OSAKA 565,JAPAN
来源
EUROPEAN JOURNAL OF NUCLEAR MEDICINE | 1996年 / 23卷 / 04期
关键词
dopamine transporter; serotonin transporter; 3 beta-(4-iodophenyl)tropane-2 beta-carboxylic acid; single-photon emission tomography;
D O I
10.1007/BF01247372
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Iodine-123-labelled 3 beta-(4-iodophenyl)tropane-2 beta-carboxylic acid ([I-123]beta-CIT) labels both the dopamine transporter (DAT) and the serotonin transporter (5-HTT) and this ligand is able to clarify pathological changes in both dopaminergic and serotonergic systems. However, the differential kinetics of beta-CIT binding to DAT and 5-HTT has not been clarified fully. In this study we examined time-activity curves of [I-123]beta-CIT in individual regions in the rat brain. Using cerebellum as the reference region, k(3) and k(4) values were estimated by a two-compartment kinetic analysis. In the striatum, the kinetics was slowest among all brain areas. In this area specific binding reached its peak 4 h after the injection. In the hypothalamus, specific binding reached its peak 1 h after the injection and its amount did not change until 4 h after the injection. In the occipital cortex, the binding and washout of the ligand were fastest among all brain regions. Estimated k(3) values were 0.040+/-0.003 in the striatum, 0.019+/-0.002 in the hypothalamus and 0.082+/-0.011 in the occipital cortex (min(-1), mean +/-SD). Estimated k(4) values were 0.0034+/-0.0005 in the striatum, 0.0071+/-0.0009 in the hypothalamus and 0.083+/-0.013 in the occipital cortex (min(-1), mean +/-SD). Therefore binding kinetics of [I-123]beta-CIT in the region rich in DAT is apparently different from that in the region rich in 5-HTT. These results will provide fundamental data to image both DAT and 5-HTT in one series of examinations with [I-123]beta-CIT.
引用
收藏
页码:431 / 436
页数:6
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