The agent of Human Granulocytic Ehrlichiosis resides in an endosomal compartment

被引:63
作者
Webster, P
IJdo, JW
Chicoine, LM
Fikrig, E
机构
[1] Yale Univ, Sch Med, Dept Internal Med, Sect Rheumatol,Lab Clin Invest 604, New Haven, CT 06520 USA
[2] Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06520 USA
关键词
Ehrlichia; intracellular pathogen; neutrophil; endosome; antibody;
D O I
10.1172/JCI1544
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The composition of cytoplasmic vacuoles containing the agent of Human Granulocytic Ehrlichiosis (HGE) was studied to investigate how this pathogen exists within infected host cells. Electron microscopy demonstrated that the HGE organism resides in a membrane-bound compartment within HL-60 cells: early forms of the HGE agent have a round reticular appearance while later structures are small and dense. Vacuoles containing HGE bacteria incorporated endocytosed colloidal gold particles, suggesting that they are part of the endocytic pathway. Antibodies directed to the mannose-6-phosphate receptor labeled vacuole membranes. Antibodies to the transferrin receptor and to the lysosomal membrane glycoprotein LAMP 1 did not. Moreover, 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine, which normally accumulates in compartments with low pH, was not present inside these vacuoles. These results suggest that vacuoles containing the agent of HGE fail to mature into phagolysosomes. We conclude that the agent of HGE appears to enter and modify part of the endocytic pathway.
引用
收藏
页码:1932 / 1941
页数:10
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