Enhancing activity and controlling stereoselectivity in a designed PLP-dependent aldolase

被引：36

作者：

Toscano, Miguel D. ^{[1
]}

Mueller, Manuel M. ^{[1
]}

Hilvert, Donald ^{[1
]}

机构：

[1] ETH, Organ Chem Lab, CH-8093 Zurich, Switzerland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 24期

关键词：

aldolases; amino acids; biocatalysis; rational design; stereoselectivity;

D O I：

10.1002/anie.200700710

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Chop and change: Site-directed mutagenesis has been employed to optimize the activity of an engineered pyridoxal phosphate-dependent aldolase and to invert its inherent threo selectivity in the cleavage of D-β-phenylserines. The modification of the active-site residues generates significant retroaldol activity that compares favorably with that of natural enzymes in terms of efficiency and selectivity. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：4468 / 4470

页数：3

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