Optical sensors for detection of bacteria. 1. General concepts and initial development

被引:38
作者
Chuang, H
Macuch, P
Tabacco, MB
机构
[1] Echo Technol Inc, Boston, MA 02210 USA
[2] Altran Corp, Boston, MA 02210 USA
关键词
D O I
10.1021/ac000459b
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The concept of using immobilized nucleic acid stains as detection chemistry to fabricate optical bacterial sensors is first demonstrated. SYTO 13 (a green fluorescent cell stain) is used as the molecular recognition element and fluorescent reporter in the sensor. The sensor responds to aqueous and aerosolized bacterial samples in 15 and 30 min, respectively. In addition, the sensor can discriminate a change in Pseudomonas aeruginosa (Pa) cell concentration of 1 order of magnitude or less and can detect down to 2.4 x 10(5) cells/mL of Pa cells. The utility of the sensor is demonstrated by monitoring the growth of a Pa cell culture over a period of 50 h.
引用
收藏
页码:462 / 466
页数:5
相关论文
共 15 条
  • [1] Infectious disease - PCR detection of bacteria in seven minutes
    Belgrader, P
    Benett, W
    Hadley, D
    Richards, J
    Stratton, P
    Mariella, R
    Milanovich, F
    [J]. SCIENCE, 1999, 284 (5413) : 449 - 450
  • [2] Immunoelectrochemical assays for bacteria: Use of epifluorescence microscopy and rapid-scan electrochemical techniques in development of an assay for Salmonella
    Brewster, JD
    Gehring, AG
    Mazenko, RS
    VanHouten, LJ
    Crawford, CJ
    [J]. ANALYTICAL CHEMISTRY, 1996, 68 (23) : 4153 - 4159
  • [3] Immunoassays based on surface enhanced infrared absorption spectroscopy
    Brown, CW
    Li, Y
    Seelenbinder, JA
    Pivarnik, P
    Rand, AG
    Letcher, SV
    Gregory, OJ
    Platek, MJ
    [J]. ANALYTICAL CHEMISTRY, 1998, 70 (14) : 2991 - 2996
  • [4] DETECTION OF YERSINIA-PESTIS FRACTION-1 ANTIGEN WITH A FIBER OPTIC BIOSENSOR
    CAO, LK
    ANDERSON, GP
    LIGLER, FS
    EZZELL, J
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (02) : 336 - 341
  • [5] Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology
    Guschin, DY
    Mobarry, BK
    Proudnikov, D
    Stahl, DA
    Rittmann, BE
    Mirzabekov, AD
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (06) : 2397 - 2402
  • [6] HAUGLAND RP, 1996, HDB FLUORESCENT PROB, P148
  • [7] Microbial detection
    Hobson, NS
    Tothill, I
    Turner, APF
    [J]. BIOSENSORS & BIOELECTRONICS, 1996, 11 (05) : 455 - 477
  • [8] HOGAN H, 1999, BIOPHOTONICS INT JUL, P18
  • [9] MITCHELL R, 1998, Patent No. 5809185
  • [10] Mittelman M. W., 1983, MICROCONTAMINATION, V1, P32