Simultaneous evaluation of dual gene transfer to adherent cells by gamma-ray imaging

被引：20

作者：

Zinn, KR ^{[1
]}

Chaudhuri, TR

Buchsbaum, DJ

Mountz, JM

Rogers, BE

机构：

[1] Univ Alabama Birmingham, Dept Radiol, Birmingham, AL 35294 USA

[2] Univ Alabama Birmingham, Dept Radiat Oncol, Birmingham, AL USA

来源：

NUCLEAR MEDICINE AND BIOLOGY | 2001年 / 28卷 / 02期

关键词：

imaging; gene transfer; somatostatin receptor; thymidine kinase; Tc-99m; I-131;

D O I：

10.1016/S0969-8051(00)00207-9

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

A gamma camera imaging method was developed to detect dual gene transfer to adherent cells growing as monolayers in cell culture plates. Human cancer cells were infected with replication-incompetent adenoviral vectors encoding the human type 2 somatostatin receptor (Ad-hSSTr2) and/or herpes virus thymidine kinase (Ad-TK). The hSSTr2 and TK reporter proteins were detected by imaging internally bound Tc-99m-P2045 peptide (Diatide, Inc.) and radioiodinated 2'-deoxy-2'-fluoro-beta -D-arabinofuranosyl-5-iodouracil (FIAU), respectively. Following gene transfer, expression of hSSTr2 and TK were accurately imaged in vitro. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：135 / 144

页数：10

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