5-LIPDXYGENASE IN MOUSE CEREBELLAR Purkinje CELLS

It has been suggested that the enzymatic pathway of 5 lipoxygenase (5 LOX) influences brain functioning and pathobiology The mRNAs for both the enzyme 5 LOX and its activating protein FLAP have been found in the cerebellum In this work, we investigated the cellular expression of 5 LOX in the adult mouse cellabellar cortex We used the in situ mRNA hybridization assay, immunocytochemistry, laser capture microdissection, and our previously developed method for as saying the DNA methylation status of a putative mouse 5 LOX promoter Since both 5-LOX mRNA in situ hybridization signal and FLAP immunoreactivity co localize with calbindin 28 kD immunoreactivity (a Purkinje cell marker) but not with S 100 beta immunoreactivity (a Bergmann glia marker), the suggestion is that the 5 LOX pathway is expressed in cerebellar Purkinje cells We found that methylation in the sites targeted by methylation sensitive restriction endonucleases Acil and HinP1I but not BstUl and Hpall was greater in DNA samples obtained from a high 5 LOX expressing cerebellar region (Purkinje cells) versus a low 5 LOX expressing region (the molecular cell layer), suggesting a possible epigenetic con tribution to the cell specific 5 LOX expression in the cerebellum We propose that Purkinje cell localized 5 LOX and FLAP expression may be involved in the cerebellar synthesis of leukotrienes and/or could influence the Dicer mediated mi croRNA formation and processes of neuroplasticity (C) 2010 IBRO Published by Elsevier Ltd All rights reserved