Comparison of two PCRs for detection of Mycobacterium ulcerans

被引：54

作者：

Guimaraes-Peres, A

Portaels, F

de Rijk, P

Fissette, K

Pattyn, SR

van Vooren, JP

Fonteyne, PA

机构：

[1] Inst Trop Med, Dept Microbiol, Mycobacteriol Unit, B-2000 Antwerp, Belgium

[2] Erasme Univ Hosp, Dept Pneumol, B-1070 Brussels, Belgium

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 01期

关键词：

D O I：

10.1128/JCM.37.1.206-208.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA gene PCR, the repetitive-sequence PCR was faster, easier to perform, and less expensive.

引用

页码：206 / 208

页数：3

共 14 条

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