Minimally invasive and selective hydrodynamic gene therapy of liver segments in the pig and human

被引：60

作者：

Khorsandi, S. E. ^{[1
]}

Bachellier, P. ^{[1
,2
]}

Weber, J. C. ^{[1
,2
]}

Greget, M. ^{[2
]}

Jaeck, D. ^{[2
]}

Zacharoulis, D. ^{[3
]}

Rountas, C. ^{[3
]}

Helmy, S. ^{[1
,4
]}

Helmy, A. ^{[4
]}

Al-Waracky, M. ^{[4
]}

Salama, H. ^{[4
]}

Jiao, L. ^{[1
]}

Nicholls, J. ^{[1
]}

Davies, A. J. ^{[1
]}

Levicar, N. ^{[1
]}

Jensen, S. ^{[1
]}

Habib, N. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Fac Med, Hammersmith Hosp, London W12 0NN, England

[2] Hosp Hautpierre, Fdn Transplantat, Strasbourg, France

[3] Larissa Univ, Sch Med, Larisa, Greece

[4] Cairo Univ, Fac Med, Cairo, Egypt

来源：

CANCER GENE THERAPY | 2008年 / 15卷 / 04期

关键词：

hydrodynamic gene therapy; human; pig;

D O I：

10.1038/sj.cgt.7701119

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This paper highlights our experience of the transfer of hydrodynamic gene therapy (HGT) from the large animal, the pig, into clinical practice. The modification of balloon catheters and the development of a minimally invasive technique to allow selective isolation of liver segments for HGT in the large animal and human are described. Finally, our preliminary results from a phase I clinical study of HGT for thrombopoietin (TPO) in cirrhotic patients with thrombocytopenia are discussed. Based on these provisional data, minimally invasive selective HGT of liver segments appears to be technically safe, but further work is required to optimize the efficiency of gene transfer in order to achieve clinical benefit.

引用

页码：225 / 230

页数：6

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