Development of a bovine X chromosome linkage group and painting probes to assess cattle, sheep, and goat X chromosome segment homologies

被引:67
作者
deLeon, FAP
Ambady, S
Hawkins, GA
Kappes, SM
Bishop, MD
Robl, JM
Beattie, CW
机构
[1] UNIV MASSACHUSETTS,GRAD PROGRAM MOL & CELLULAR BIOL,PAIGE LAB,AMHERST,MA 01003
[2] USDA ARS,ROMAN L HRUSKA US MEAT ANIM RES CTR,CLAY CTR,NE 68933
关键词
D O I
10.1073/pnas.93.8.3450
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The X chromosome linkage group is conserved in placental mammals, However, X chromosome morphological differences, due to internal chromosome rearrangements, exist among mammalian species, We have developed bovine chromosome painting probes for Xp and Xq to assess segment homologies between the submetacentric bovine X chromosome and the acrocentric sheep and goat X chromosomes, These painting probes and their corresponding DNA libraries were developed by chromosome micromanipulation, DNA micropurification, microcloning, and PCR amplification, The bovine Xp painting probe identified an interstitially located homologous segment in the sheep and goat Xq region, most probably resulting from chromosome inversion. Ten type II (microsatellite) markers obtained from the bovine Xq library and five other X chromosome assigned, but unlinked, markers were used to generate a linkage map for Xq spanning 89.4 centimorgans. The chromosome painting probes and molecular markers generated in this study would be useful for comparative mapping and tracing of internal X chromosome rearrangements in all ruminant species and would contribute to the understanding of mammalian sex chromosome evolution.
引用
收藏
页码:3450 / 3454
页数:5
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