Fructans and fructan-metabolizing enzymes in leaves of Lolium perenne

被引:47
作者
Pavis, N [1 ]
Boucaud, J [1 ]
Prud'homme, MP [1 ]
机构
[1] Univ Caen, INRA, UA, Lab Physiol & Biochim Vegetales, F-14032 Caen, France
关键词
fructans; fructan : fructan 6G-fructosyltransferase; Lolium; synthesis;
D O I
10.1046/j.1469-8137.2001.00068.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Longitudinal variability is reported in internal carbon partitioning as is the activity of enzymes involved in fructan synthesis during leaf development of Lolium perenne cv. Bravo. Sink activity was reduced in L. perenne plants by cooling the roots while continuous illumination enhanced source activity, resulting in preferential accumulation of fructans at the leaf base. Fructan contents and enzymatic activity of two trisaccharides (SST (sucrose:sucrose fructosyltransferase) and 6G-FT (fructan:fructan 6C-fructosyltransferase)) were measured in segments of elongating leaves, both mature leaf blades and sheaths. The complement of fructans accumulated was similar in both elongating leaves and mature leaf sheaths. 6G-FT activity was found in L. perenne and characterized in a crude extract; this activity was greatest in the basal segment of elongating leaves and mature leaf sheaths. Throughout the leaf blade, however, SST and 6G-FT activities remained low and constant, and did not increase under conditions of sucrose accumulation. Differences are shown in fructan accumulation between leaf tissues; the significance of these findings and the putative pathway of fructan synthesis in L perenne are discussed.
引用
收藏
页码:97 / 109
页数:13
相关论文
共 44 条
[1]   PHOTOSYNTHATE PARTITIONING IN BASAL ZONES OF TALL FESCUE LEAF BLADES [J].
ALLARD, G ;
NELSON, CJ .
PLANT PHYSIOLOGY, 1991, 95 (03) :663-668
[2]   PURIFICATION AND CHARACTERIZATION OF FRUCTANS WITH BETA-2,1-GLYCOSIDIC AND BETA-2,6-GLYCOSIDIC LINKAGES SUITABLE FOR ENZYME STUDIES [J].
BONNETT, GD ;
SIMS, IM ;
STJOHN, JA ;
SIMPSON, RJ .
NEW PHYTOLOGIST, 1994, 127 (02) :261-269
[3]   COMPARTMENTATION AND FLUXES OF CARBON IN LEAF BLADES AND LEAF SHEATHS OF POA-ANNUA L AND POA X JEMTLANDICA (ALMQ) RICHT [J].
BORLAND, AM ;
FARRAR, JF .
PLANT CELL AND ENVIRONMENT, 1988, 11 (06) :535-543
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   THE INTERPRETATION OF INVITRO MEASUREMENTS OF FRUCTOSYL TRANSFERASE-ACTIVITY - AN ANALYSIS OF PATTERNS OF FRUCTOSYL TRANSFER BY FUNGAL INVERTASE [J].
CAIRNS, AJ ;
ASHTON, JE .
NEW PHYTOLOGIST, 1991, 118 (01) :23-34
[6]   Fructan biosynthesis in excised leaves of Lolium temulentum [J].
Cairns, AJ ;
Bonnett, GD ;
Gallagher, JA ;
Simpson, RJ ;
Pollock, CJ .
NEW PHYTOLOGIST, 1997, 136 (01) :61-72
[7]   A RECONSIDERATION OF FRUCTAN BIOSYNTHESIS IN STORAGE ROOTS OF ASPARAGUS-OFFICINALIS L [J].
CAIRNS, AJ .
NEW PHYTOLOGIST, 1992, 120 (04) :463-473
[8]  
CAIRNS AJ, 1993, NEW PHYTOL, V123, P15, DOI 10.1111/j.1469-8137.1993.tb04526.x
[9]   FRUCTAN BIOSYNTHESIS IN EXCISED LEAVES OF LOLIUM TEMULENTUM L .1. CHROMATOGRAPHIC CHARACTERIZATION OF OLIGOFRUCTANS AND THEIR LABELING PATTERNS FOLLOWING (CO2)-C-14 FEEDING [J].
CAIRNS, AJ ;
POLLOCK, CJ .
NEW PHYTOLOGIST, 1988, 109 (04) :399-405
[10]   Characterization of the enzymatic polymerization of 2,6-linked fructan by leaf extracts from timothy grass (Phleum pratense) [J].
Cairns, AJ ;
Nash, R ;
De Carvalho, MAM ;
Sims, IM .
NEW PHYTOLOGIST, 1999, 142 (01) :79-91