Enhanced antibody and cytokine responses to influenza viral antigens in perforin-deficient mice

被引:31
作者
Sambhara, S [1 ]
Switzer, I [1 ]
Kurichh, A [1 ]
Miranda, R [1 ]
Urbanczyk, L [1 ]
James, O [1 ]
Underdown, B [1 ]
Klein, M [1 ]
Burt, D [1 ]
机构
[1] Pasteur Merieux Connaught Canada, Res Ctr, N York, ON M2R 3T4, Canada
关键词
D O I
10.1006/cimm.1998.1314
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cytotoxic T lymphocytes (CTL) lyse virus-infected target cells by secreting the pore-forming effector molecule, perforin. Perforin-mediated cell death appears to be a major mechanism in viral clearance but its role in regulating immune responses in vivo is unclear. In this report, we show that following immunization with influenza viral antigens, perforin-deficient mice generated about 100-fold greater serum antibody responses than wild-type mice. Further, immune spleen cells from perforin knock-out mice secreted over 10-fold more IFN-gamma following in vitro restimulation than immune spleen cells from control mice. Finally, there were over 10-fold more IFN-gamma-secreting cells in cultures from perforin-deficient mice than those from control mice, suggesting that the enhanced cytokine release by T cells from perforin-deficient mice is due to an increase in the effector cell pool. Collectively, these results suggest that perforin-mediated effector function is required in the down-regulation of the immune response by way of limiting antigen-presenting cell function. (C) 1998 Academic Press.
引用
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页码:13 / 18
页数:6
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