Soluble proteins of the nacre of the giant oyster Pinctada maxima and of the abalone Haliotis tuberculata:: extraction and partial analysis of nacre proteins

被引:56
作者
Bédouet, L
Schuller, MJ
Marin, F
Milet, C
Lopez, E
Giraud, M
机构
[1] Museum Natl Hist Nat, CNRS, UMR 8572, Lab Physiol Gen & Comparee, F-75231 Paris 05, France
[2] MNHN, Stat Biol Marine, F-29182 Concarneau, France
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2001年 / 128卷 / 03期
关键词
oyster; abalone; nacre; proteins; conchiolin; glycoproteins; stains-all; Alcian blue;
D O I
10.1016/S1096-4959(00)00337-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Several proteins from nacre of the oyster Pinctada maxima and the abalone Haliotis tuberculata were extracted and partly characterized. Proteins dispersed in aragonite were solubilized during demineralization with acetic acid whereas proteins adsorbed on conchiolin were extracted with sodium dodecyl sulfate and beta -mercaptoethanol. The matrix of Pinctada maxima nacre is composed of one main protein with an apparent molecular weight of 20 kDa (p20). This protein was found in the acetic acid soluble fraction of nacre, as well as in the Laemmli-solubilized extract of conchiolin. In addition, the p20 solubilized with acetic acid can form oligomers made of 6 monomers linked together by disulfide bridges. The first N-terminal 21 amino acids of p20 were determined and no homology with known proteins was found. In Haliotis tuberculata nacre, 5 main proteins were solubilized during demineralization and 3 glycoproteins were detected. Stains-all and Alcian blue staining revealed polyanionic proteins in the extracts isolated from Pinctada maxima and Haliotis tuberculata nacre. (C) 2001 Elsevier Science Inc. All rights reserved.
引用
收藏
页码:389 / 400
页数:12
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