Temperature gradient focusing in a PDMS/glass hybrid microfluidic chip

被引：46

作者：

Matsui, Takuya ^{[1
,2
]}

Franzke, Joachim ^{[1
]}

Manz, Andreas ^{[1
]}

Janasek, Dirk ^{[1
]}

机构：

[1] ISAS Inst Analyt Sci Dortmund & Berlin, D-44139 Dortmund, Germany

[2] Hitachi High Technol Corp, Nanotechnol Prod Business Grp, Biomed Ctr, Div Res & Dev, Ibaraki, Japan

来源：

ELECTROPHORESIS | 2007年 / 28卷 / 24期

关键词：

EOF; PDMS; miniaturization; sample concentration techniques; temperature gradient focusing;

D O I：

10.1002/elps.200700272

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper reports the application of temperature gradient focusing (TGF) in a PDMS/glass hybrid microfluidic chip. With TGF, by the combination of a temperature gradient along a microchannel, an applied electric field, and a buffer with a temperature-dependent ionic strength, analytes are focused by balancing their electrophoretic velocities against the bulk velocity of the buffer containing the analytes. In this work, Oregon Green 488 carboxylic acid was concentrated approximately 30 times as high as the initial concentration in 45 s at moderate electric strength of 70 V/cm and a temperature gradient of 55 degrees C across the PDMS/glass hybrid microfluidic chip with a 1 cm long capillary.

引用

页码：4606 / 4611

页数：6

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