Interaction of lysozyme with negatively charged flexible chain polymers

被引：49

作者：

Romanini, Diana

Braia, Mauricio

Angarten, Rodrigo Giatte

Loh, Watson

Pico, Guillermo

机构：

[1] Natl Univ Rosario, Fac Biochem & Pharmaceut Sci, Bioseperat Lab, Dept Chem Phys,FonCyt,CIUNR, Rosario, Argentina

[2] Consejo Nacl Invest Cient & Tecn, Rosario, Argentina

[3] Univ Estadual Campinas, Inst Chem, Campinas, SP, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2007年 / 857卷 / 01期

关键词：

lysozyme; poly vinyl sulfonate; poly acrylic acid; protein-polyelectrolyte complex;

D O I：

10.1016/j.jchromb.2007.06.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The complex formation between the basic protein lysozyme and anionic polyelectrolytes: poly acrylic acid and poly vinyl sulfonic acid was studied by turbidimetric and isothermal calorimetric titrations. The thermodynamic stability of the protein in the presence of these polymers was also studied by differential scanning calorimetry. The lysozyme-polymer complex was insoluble at pH lower than 6, with a stoichiometric ratio (polymer per protein mol) of 0.025-0.060 for lysozyme-poly vinyl sulfonic acid and around 0.003-0.001 for the lysozyme-poly acrylic acid. NaCl 0.1 M inhibited the complex precipitation in agreement with the proposed coulombic mechanism of complex formation. Enthalpic and entropic changes associated to the complex formation showed highly negative values in accordance with a coulombic interaction mechanism. The protein tertiary structure and its thermodynamic stability were not affected by the presence of polyclectrolyte. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：25 / 31

页数：7

共 19 条

[1]

Calorimetric and structural investigation of the interaction of lysozyme and bovine serum albumin with poly(ethylene oxide) and its copolymers [J].

Almeida, NL ;

Oliveira, CLP ;

Torriani, IL ;

Loh, W .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2004, 38 (1-2) :67-76

[2]

Reversible precipitation of proteins on carboxymethyl cellulose [J].

Lali, A ;

Aruna, N ;

John, R ;

Thakrar, D .

PROCESS BIOCHEMISTRY, 2000, 35 (08) :777-785

[3]

Formation of an intrapolymer complex from human serum albumin and poly(ethylene glycol) [J].

Azegami, S ;

Tsuboi, A ;

Izumi, T ;

Hirata, M ;

Dubin, PL ;

Wang, BL ;

Kokufuta, E .

LANGMUIR, 1999, 15 (04) :940-947

[4]

Polyelectrolyte-protein complexes [J].

Cooper, CL ;

Dubin, PL ;

Kayitmazer, AB ;

Turksen, S .

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 2005, 10 (1-2) :52-78

[5]

Molecular thermodynamics of precipitation [J].

Fornasiero, F ;

Ulrich, J ;

Prausnitz, JM .

CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION, 1999, 38 (4-6) :463-475

[6]

van't Hoff and calorimetric enthalpies from isothermal titration calorimetry: Are there significant discrepancies? [J].

Horn, JR ;

Russell, D ;

Lewis, EA ;

Murphy, KP .

BIOCHEMISTRY, 2001, 40 (06) :1774-1778

[7]

Polycomplexes - potential for bioseparation [J].

Izumrudov, VA ;

Galaev, IY ;

Mattiasson, B .

BIOSEPARATION, 1998, 7 (4-5) :207-220

[8]

Complex formation of protein with different water-soluble synthetic polymers [J].

Matsudo, T ;

Ogawa, K ;

Kokufuta, E .

BIOMACROMOLECULES, 2003, 4 (06) :1794-1799

[9]

Complex formation between bovine serum albumin and strong polyelectrolytes: Effect of polymer charge density [J].

Mattison, KW ;

Dubin, PL ;

Brittain, IJ .

JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (19) :3830-3836

[10]

Host selection as a downstream strategy:: Polyelectrolyte precipitation of β-glucuronidase from plant extracts [J].

Menkhaus, TJ ;

Eriksson, SU ;

Whitson, PB ;

Glatz, CE .

BIOTECHNOLOGY AND BIOENGINEERING, 2002, 77 (02) :148-154

← 1 2 →