Chloroplast-derived vaccine antigens and other therapeutic proteins

被引：88

作者：

Daniell, H ^{[1
]}

Chebolu, S ^{[1
]}

Kumar, S ^{[1
]}

Singleton, M ^{[1
]}

Falconer, R ^{[1
]}

机构：

[1] Univ Cent Florida, Dept Mol Biol & Microbiol, Orlando, FL 32816 USA

来源：

VACCINE | 2005年 / 23卷 / 15期

关键词：

chloroplast genetic engineering; plant made biopharmaceuticals; oral delivery;

D O I：

10.1016/j.vaccine.2004.11.004

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The chloroplast genetic engineering offers a number of unique advantages including high level of transgene expression, multi-gene expression in single transformation event and transgene containment due to maternal inheritance. Hyper-expression of vaccine antigens or therapeutic proteins in transgenic chloroplasts (leaves) or chromoplasts (fruits/roots) facilitates efficient oral delivery. Ability of chloroplasts to correctly fold human blood proteins with proper disulfide bridges (human serum albumin or interferons) or presence of chaperones in chloroplasts to facilitate assembly of complex multi-subunit proteins or their prokaryotic nature to express native bacterial genes (up to 46.1% total leaf protein) are attractive features for therapeutic protein production. Purification of therapeutic proteins has been achieved using novel purification strategies that do not require expensive column chromatography. (c) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1779 / 1783

页数：5

共 16 条

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