Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges

被引：45

作者：

Lei, Ting

Jacob, Sandrine

Ajil-Zaraa, Imen

Dubuisson, Jean-Bernard

Irion, Olivier

Jaconi, Marisa

Feki, Anis ^{[1
]}

机构：

[1] Univ Hosp Geneva, Dept Obstet & Gynecol, Lab Stem Cell Res, Geneva, Switzerland

[2] Univ Hosp Geneva, Dept Obstet & Gynecol, Geneva, Switzerland

[3] Univ Geneva, Fac Med, Dept Pathol & Immunol, Geneva, Switzerland

来源：

CELL RESEARCH | 2007年 / 17卷 / 08期

关键词：

embryonic stem cells; inner cell mass; derivation; feeder cells; xeno-free; differentiation;

D O I：

10.1038/cr.2007.61

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Human embryonic stem cells (hESC) not only hold great promise for the treatment of degenerative diseases but also provide a valuable tool for developmental studies. However, the clinical applications of hESC are at present limited by xeno-contamination during the in vitro derivation and propagation of these cells. In this review, we summarize the current methodologies for the derivation and the propagation of hESC in conditions that will eventually enable the generation of clinical-grade cells for future therapeutic applications.

引用

收藏

页码：682 / 688

页数：7

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