Tobacco plants perturbed in the ubiquitin-dependent protein degradation system accumulate callose, salicylic acid, and pathogenesis-related protein 1

被引:22
作者
Conrath, U
Klessig, DF
Bachmair, A [1 ]
机构
[1] Univ Vienna, Inst Bot, A-1030 Vienna, Austria
[2] Rutgers State Univ, Waksman Inst, Piscataway, NJ 08855 USA
[3] Rutgers State Univ, Dept Mol Biol & Biochem, Piscataway, NJ 08855 USA
基金
美国国家科学基金会;
关键词
lesion mimic; hypersensitive response; ubiquitin-dependent protein degradation ubiquitin variant; tobacco;
D O I
10.1007/s002990050501
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Transgenic tobacco plants expressing an inhibitor of ubiquitin-dependent protein degradation, ubiquitin variant ubR48, spontaneously formed necrotic lesions and displayed altered responsiveness to tobacco mosaic virus attack. These plants were analyzed for the accumulation of defense-related compounds and the expression of pathogenesis-related proteins, which serve as convenient markers for systemic acquired resistance. Callose was detected in the cells of vascular bundles and in the leaf blade. In addition, ubR48 transgenic plants constitutively accumulated enhanced levels of salicylic acid (SA) and/or its glucoside. Accumulation of SA glucoside coincided with high levels of pathogenesis-related protein 1, underscoring the similarity of certain changes in ubR48-expressing plants to an authentic defense reaction.
引用
收藏
页码:876 / 880
页数:5
相关论文
共 43 条
  • [1] Structure and development of the tobacco leaf
    Avery, GS
    [J]. AMERICAN JOURNAL OF BOTANY, 1933, 20 (09) : 565 - 592
  • [2] PERTURBATION OF THE UBIQUITIN SYSTEM CAUSES LEAF CURLING, VASCULAR TISSUE ALTERATIONS AND NECROTIC LESIONS IN A HIGHER-PLANT
    BACHMAIR, A
    BECKER, F
    MASTERSON, RV
    SCHELL, J
    [J]. EMBO JOURNAL, 1990, 9 (13) : 4543 - 4549
  • [3] BACHMAIR A, 1994, ADV MOL GENETICS PLA, V3, P375
  • [4] NF-kappa B: Ten years after
    Baeuerle, PA
    Baltimore, D
    [J]. CELL, 1996, 87 (01) : 13 - 20
  • [5] ALTERED RESPONSE TO VIRAL-INFECTION BY TOBACCO PLANTS PERTURBED IN UBIQUITIN SYSTEM
    BECKER, F
    BUSCHFELD, E
    SCHELL, J
    BACHMAIR, A
    [J]. PLANT JOURNAL, 1993, 3 (06) : 875 - 881
  • [6] CHOLERA-TOXIN ELEVATES PATHOGEN RESISTANCE AND INDUCES PATHOGENESIS-RELATED GENE-EXPRESSION IN TOBACCO
    BEFFA, R
    SZELL, M
    MEUWLY, P
    PAY, A
    VOGELILANGE, R
    METRAUX, JP
    NEUHAUS, G
    MEINS, F
    NAGY, F
    [J]. EMBO JOURNAL, 1995, 14 (23) : 5753 - 5761
  • [7] An essential role for NF-kappa B in preventing TNF-alpha-induced cell death
    Beg, AA
    Baltimore, D
    [J]. SCIENCE, 1996, 274 (5288) : 782 - 784
  • [8] A MUTATION IN ARABIDOPSIS THAT LEADS TO CONSTITUTIVE EXPRESSION OF SYSTEMIC ACQUIRED-RESISTANCE
    BOWLING, SA
    GUO, A
    CAO, H
    GORDON, AS
    KLESSIG, DF
    DONG, XI
    [J]. PLANT CELL, 1994, 6 (12) : 1845 - 1857
  • [9] The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance
    Bowling, SA
    Clarke, JD
    Liu, YD
    Klessig, DF
    Dong, XN
    [J]. PLANT CELL, 1997, 9 (09) : 1573 - 1584
  • [10] Plant pathology: Many roads lead to resistance
    Boyes, DC
    McDowell, JM
    Dangl, JL
    [J]. CURRENT BIOLOGY, 1996, 6 (06) : 634 - 637