Studies on fluorescence resonance energy transfer between dyes and water-soluble quantum dots

被引：40

作者：

Chen, QD ^{[1
]}

Ma, QA ^{[1
]}

Wan, Y ^{[1
]}

Su, XG ^{[1
]}

Lin, ZB ^{[1
]}

Jin, QH ^{[1
]}

机构：

[1] Jilin Univ, Dept Analyt Chem, Coll Chem, Changchun 130023, Peoples R China

来源：

LUMINESCENCE | 2005年 / 20卷 / 4-5期

关键词：

quantum dots; fluorescence resonance energy transfer; organic dyes;

D O I：

10.1002/bio.840

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, donor-acceptor complexes were formed based on antibody-antigen interactions. Immunoglobulin antigen (mouse-IgG) was effectively conjugated to mercaptopropyl acid-modified CdTe quantum dot synthesized in aqueous solution via electrostatic interaction, while organic dyes-tetramethylrhodamine isothiocyanate (TRITC) were attached to the corresponding antibody (anti-mouse IgG). The mutual affinity of the antigen and antibody brought the CdTe quantum dot and TRITC sufficiently close together to allow the resonance dipole-dipole coupling required for fluorescence resonance energy transfer to occur. The formation of immunocomplexes resulted in fluorescence resonance energy transfer from the CdTe quantum dot donors to the TRITC acceptors. Copyright (c) 2005 John Wiley & Sons, Ltd.

引用

页码：251 / 255

页数：5

共 21 条

[1] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[2] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[3]

CHEN GZ, 1990, FLUORESCENT SPECTROM

[4] Enhanced Forster energy transfer in organic/inorganic bilayer optical microcavities [J].

Finlayson, CE ;

Ginger, DS ;

Greenham, NC .

CHEMICAL PHYSICS LETTERS, 2001, 338 (2-3) :83-87

[5] Strongly photoluminescent CdTe nanocrystals by proper surface modification [J].

Gao, MY ;

Kirstein, S ;

Möhwald, H ;

Rogach, AL ;

Kornowski, A ;

Eychmüller, A ;

Weller, H .

JOURNAL OF PHYSICAL CHEMISTRY B, 1998, 102 (43) :8360-8363

[6] Limitations arising from optical saturation in fluorescence and thermal lens spectrometries using pulsed laser excitation: Application to the determination of the fluorescence quantum yield of rhodamine 6G [J].

Georges, J ;

Arnaud, N ;

Parise, L .

APPLIED SPECTROSCOPY, 1996, 50 (12) :1505-1511

[7] Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules [J].

Han, MY ;

Gao, XH ;

Su, JZ ;

Nie, S .

NATURE BIOTECHNOLOGY, 2001, 19 (07) :631-635

[8] Long-term multiple color imaging of live cells using quantum dot bioconjugates [J].

Jaiswal, JK ;

Mattoussi, H ;

Mauro, JM ;

Simon, SM .

NATURE BIOTECHNOLOGY, 2003, 21 (01) :47-51

[9] Electronic energy transfer in CdSe quantum dot solids [J].

Kagan, CR ;

Murray, CB ;

Nirmal, M ;

Bawendi, MG .

PHYSICAL REVIEW LETTERS, 1996, 76 (09) :1517-1520

[10] Long-range resonance transfer of electronic excitations in close-packed CdSe quantum-dot solids [J].

Kagan, CR ;

Murray, CB ;

Bawendi, MG .

PHYSICAL REVIEW B, 1996, 54 (12) :8633-8643

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