Molecular identification of Bacteria and Eukarya inhabiting an Antarctic cryoconite hole

被引：190

作者：

Christner, BC ^{[1
]}

Kvitko, BH ^{[1
]}

Reeve, JN ^{[1
]}

机构：

[1] Ohio State Univ, Dept Microbiol, Columbus, OH 43210 USA

来源：

EXTREMOPHILES | 2003年 / 7卷 / 03期

基金：

美国国家科学基金会;

关键词：

Antarctica; cryoconite hole; frozen ecosystem; glacial ice; McMurdo Dry Valley; refuge survival;

D O I：

10.1007/s00792-002-0309-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inhabitants of a cryoconite hole formed in the Canada Glacier in the McMurdo Dry Valley region of Antarctica have been isolated and identified by small subunit (16S/18S) rDNA amplification, cloning, and sequencing. The sequences obtained revealed the presence of members of eight bacterial lineages (Acidobacterium, Actinobacteria, Cyanobacteria, Cytophagales, Gemmimonas, Planctomycetes, Proteobacteria, and Verrucomicrobia) and metazoan (nematode, tardigrade, and rotifer), truffle (Choiromyces), ciliate (Spathidium), and green algal (Pleurastrium) Eukarya. Bacterial recovery was similar to20-fold higher at 4 degreesC and 15 degreesC than at 22 degreesC, and obligately psychrophilic bacteria were identified and isolated. Several of the rDNA molecules amplified from isolates and directly from cryoconite DNA preparations had sequences similar to rDNA molecules of species present in adjacent lake ice and microbial mat environments. This cryoconite hole community was therefore most likely seeded by particulates from these local environments. Cryoconite holes may serve as biological refuges that, on glacial melting, can repopulate the local environments.

引用

页码：177 / 183

页数：7

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