Roles of Rho/ROCK and MLCK in TNF-α-induced changes in endothelial morphology and permeability

被引:215
作者
Mckenzie, Jenny A. G.
Ridley, Anne J.
机构
[1] Royal Free & Univ Coll Sch Med, Ludwig Inst Canc Res, London, England
[2] UCL, Dept Biochem & Mol Biol, London, England
关键词
D O I
10.1002/jcp.21114
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Tumor necrosis factor-alpha (TNF-alpha) is known to induce changes in endothelial cell morphology and permeability, but the mechanisms have not been extensively characterized. TNF-alpha rapidly incluced RhoA activation and myosin light chain phosphorylation, but caused only small changes to cortical F-actin, without significantly increasing paracellular permeability up to 30 min after stimulation. TNF-alpha subsequently caused a progressive increase in permeability and in stress fiber reorganization, cell elongation, and intercellular gap formation over 8-24 h. Consistent with the increased permeability, Occludin and JAM-A were removed from tight junctions and ZO-I was partially redistributed. Rho/ROCK but not MLCK inhibition prevented the long-term TNF-alpha-induced changes in F-actin and cell morphology, but ROCK inhibition did not affect permeability. These results suggest that the gradual increase in permeability induced by TNF-alpha does not reflect contractile mechanisms mediated by Rho, ROCK, and MLCK, but involves long-term reorganization of tight junction proteins.
引用
收藏
页码:221 / 228
页数:8
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