Complementarity of GC-MS and LC-MS analyses for determination of carbohydrate profiles of vegetative cells and spores of bacilli

被引:20
作者
Fox, KF
Wunschel, DS
Fox, A [1 ]
Stewart, GC
机构
[1] Univ S Carolina, Sch Med, Dept Microbiol & Immunol, Columbia, SC 29208 USA
[2] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA
关键词
mass spectrometry; chromatography; bacilli; carbohydrates; teichoic acids;
D O I
10.1016/S0167-7012(98)00030-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Gas chromatography-mass spectrometry (GC-MS) and high-performance anion-exchange (HPAE) liquid chromatography-mass spectrometry (LC-MS) provide complementary information when used to characterize whole-cell carbohydrate profiles. Neutral and aminosugars are profiled (when analyzed as alditol acetates) by GC-MS. Whilst neutral and acidic sugars are detected in native form by HPAE LC-MS. The power of these complementary approaches is particularly well illustrated in the analysis of B. subtilis and the genetically related organism B. atrophaeus. Vegetative cells grown under phosphate-rich conditions, vegetative cells grown under phosphate-limiting conditions and spores are quite distinct in their carbohydrate composition. The totality of these six (three GC-MS and three LC-MS) carbohydrate profiles for each organism provides a powerful tool for characterization of the physiological status of the cell and for species identification. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:1 / 11
页数:11
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