Three CCT domain-containing genes were identified to regulate heading date by candidate gene-based association mapping and transformation in rice

被引：72

作者：

Zhang, Li ^{[1
,2
]}

Li, Qiuping ^{[1
,2
]}

Dong, Haijiao ^{[1
,2
]}

He, Qin ^{[1
,2
]}

Liang, Liwen ^{[1
,2
]}

Tan, Cong ^{[1
,2
]}

Han, Zhongmin ^{[1
,2
]}

Yao, Wen ^{[1
,2
]}

Li, Guangwei ^{[1
,2
]}

Zhao, Hu ^{[1
,2
]}

Xie, Weibo ^{[1
,2
]}

Xing, Yongzhong ^{[1
,2
,3
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

[2] Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China

[3] Yangtz Univ, Hubei Collaborat Innovat Ctr Grain Ind, Jinzhou 43434025, Peoples R China

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

基金：

中国国家自然科学基金;

关键词：

PSEUDO-RESPONSE-REGULATOR; QUANTITATIVE TRAIT LOCUS; GENOME-WIDE ASSOCIATION; CONSTANS-LIKE GENE; LINKAGE DISEQUILIBRIUM; PHOTOPERIOD SENSITIVITY; NATURAL VARIATION; ARABIDOPSIS; TIME; EXPRESSION;

D O I：

10.1038/srep07663

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

CCT domain-containing genes generally control flowering in plants. Currently, only six of the 41 CCT family genes have been confirmed to control flowering in rice. To efficiently identify more heading date-related genes from the CCT family, we compared the positions of heading date QTLs and CCT genes and found that 25 CCT family genes were located in the QTL regions. Association mapping showed that a total of 19 CCT family genes were associated with the heading date. Five of the seven associated genes within QTL regions and two of four associated genes outside of the QTL regions were confirmed to regulate heading date by transformation. None of the seven non-associated genes outside of the QTL regions regulates heading date. Obviously, combination of candidate gene-based association mapping with linkage analysis could improve the identification of functional genes. Three novel CCT family genes, including one non-associated (OsCCT01) and two associated genes (OsCCT11 and OsCCT19) regulated the heading date. The overexpression of OsCCT01 delayed flowering through suppressing the expression of Ehd1, Hd3a and RFT1 under both long day and short day conditions. Potential functions in regulating heading date of some untested CCT family genes were discussed.

引用

页数：11

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