Development of cell culture (MDCK) live cold-adapted (CA) attenuated influenza vaccine

被引：36

作者：

Ghendon, YZ

Markushin, SG

Akopova, II

Koptiaeva, IB

Nechaeva, EA

Mazurkova, LA

Radaeva, IF

Kolokoltseva, TD

机构：

[1] Res Inst Virus Preparat, Moscow 115088, Russia

[2] State Sci Ctr Vector, Cell Culture Res Inst, Koltsov 630559, Novorsibirsk Re, Russia

来源：

VACCINE | 2005年 / 23卷 / 38期

关键词：

influenza; live vaccines; MDCK cells; fermenter;

D O I：

10.1016/j.vaccine.2005.04.039

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Optimal conditions are determined for growing cold-adapted reassortant strains of a live influenza vaccine in MDCK cell line cultivated in a fermenter with a serum-free medium and microcarriers. The studied MDCK cell line meet all national and WHO requirements for the finite cell lines used for the production of biological preparations. CA reassortant vaccine strains grown in such conditions which fully preserve its mutations and the mutations lead to amino acid substitution in all genome segments of the studied CA reassortants. Under optimal cultivation conditions, the output of a monovalent live CA influenza vaccine in a 10-1 fermenter may reach 100,000 doses. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：4678 / 4684

页数：7

共 24 条

[1]

Brands R, 1999, Dev Biol Stand, V98, P93

[2] Humoral and cell-mediated immunity to Vero cell-derived influenza vaccine [J].