Fast analysis of antibacterial isothiazolones by capillary electrophoresis

被引:3
作者
Barták, P
Bednár, P
Friedecky, D
Haviger, A
Sevcík, J
机构
[1] Palacky Univ, Ctr Bioanalyt Res, Olomouc 77146, Czech Republic
[2] Palacky Univ, Dept Analyt Chem, Olomouc 77146, Czech Republic
来源
JOURNAL OF CHROMATOGRAPHY B | 2001年 / 758卷 / 02期
关键词
isothiazolones;
D O I
10.1016/S0378-4347(01)00198-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Some technical aspects influencing the total time of CE analysis are discussed. A high throughput electrophoretic system based on micellar electrokinetic chromatography (MEKC) is demonstrated as an example. A short capillary, strong electric field, alkaline buffer (pH 9.5) generating strong electroosmotic flow, and parallel hydrodynamic pressure allow the separation of two uncharged isothiazolone derivatives within 45 s. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:323 / 325
页数:3
相关论文
共 15 条
  • [1] Overview of capillary electrophoresis and capillary electrochromatography
    Altria, KD
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 856 (1-2) : 443 - 463
  • [2] Fast chiral separations using sulfated β-cyclodextrin and short-end injection in capillary electrophoresis
    Bergholdt, AB
    Jorgensen, KW
    Wendel, L
    Lehmann, SV
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2000, 875 (1-2) : 403 - 410
  • [3] CHEN FTA, 1991, CLIN CHEM, V37, P14
  • [4] Foret F., 1993, CAPILLARY ZONE ELECT
  • [5] Determination of rhodanese enzyme activity by capillary zone electrophoresis
    Glatz, Z
    Bouchal, P
    Janiczek, O
    Mandl, M
    Cesková, P
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1999, 838 (1-2) : 139 - 148
  • [6] Capillary electrophoresis for clinical problem solving: Analysis of urinary diagnostic metabolites and serum proteins
    Jellum, E
    Dollekamp, H
    Blessum, C
    [J]. JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 683 (01): : 55 - 65
  • [7] Capillary electrophoresis as a clinical tool
    Jenkins, MA
    Guerin, MD
    [J]. JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS, 1996, 682 (01): : 23 - 34
  • [8] KLEIN GL, 1994, HDB CAPILLARY ELECTR, P420
  • [9] Fast detection of a (CA)18 microsatellite repeat in the IgE receptor gene by capillary electrophoresis with laser-induced fluorescence detection
    Klepárník, K
    Malá, Z
    Havác, Z
    Blazková, M
    Hollá, L
    Bocek, P
    [J]. ELECTROPHORESIS, 1998, 19 (02) : 249 - 255
  • [10] Klepárník K, 2000, ELECTROPHORESIS, V21, P238, DOI 10.1002/(SICI)1522-2683(20000101)21:1<238::AID-ELPS238>3.0.CO