Photoelectric measurements of s-BLM/nucleoli: a new technique for studying apoptosis

被引：10

作者：

Feng, J ^{[1
]}

Zhang, CY

Ottova, AL

Tien, HT

机构：

[1] Tsing Hua Univ, Dept Chem, Beijing 100084, Peoples R China

[2] Tsing Hua Univ, Dept Phys, Beijing 100084, Peoples R China

[3] Michigan State Univ, E Lansing, MI 48824 USA

来源：

BIOELECTROCHEMISTRY | 2000年 / 51卷 / 02期

基金：

中国国家自然科学基金;

关键词：

BLMs; nucleoli; photoelectric effects; apoptosis; Taxol;

D O I：

10.1016/S0302-4598(00)00071-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method based on photoelectric measurement for analyzing apoptosis of cell-free MCF-7 nucleoli is reported. Supported bilayer lipid membrane (s-BLM) was used to enclose nucleoli in biological environment. The s-BLM was self-assembled on the wall of a super-thin cell. During the apoptosis induced by Taxol, the photoelectric current of the self-assembled s-BLM/nucleoli was found decreasing with time, suggesting the degradation of nucleus DNA. Electron transfer along the DNA double helix and along nuclear skeleton is assumed in the interpretation. This novel photoelectric analytical method may provide a rapid and sensitive technique to evaluate apoptosis. (C) 2000 Elsevier Science S.A. All rights reserved.

引用

页码：187 / 191

页数：5

共 23 条

[1] DNA-MEDIATED PHOTOELECTRON TRANSFER-REACTIONS [J].

BARTON, JK ;

KUMAR, CV ;

TURRO, NJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (20) :6391-6393

[2] DYNAMICS OF ELECTRON-TRANSFER BETWEEN INTERCALATED POLYCYCLIC MOLECULES - EFFECT OF INTERSPERSED BASES [J].

BRUN, AM ;

HARRIMAN, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (10) :3656-3660

[3] Photoelectric behavior of mammalian cells and its bioanalytical applications [J].

Ci, YX ;

Zhang, CY ;

Feng, J .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1998, 45 (02) :247-251

[4] ELECTRON CONDUCTION AND TRAPPING IN DNA - AN ELECTRON-SPIN-RESONANCE STUDY [J].

CULLIS, PM ;

MCCLYMONT, JD ;

SYMONS, MCR .

JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1990, 86 (03) :591-592

[5]

DUCK RC, 1983, P NATL ACAD SCI USA, V80, P6361

[6] INVOLVEMENT OF AN ICE-LIKE PROTEASE IN FAS-MEDIATED APOPTOSIS [J].

ENARI, M ;

HUG, H ;

NAGATA, S .

NATURE, 1995, 375 (6526) :78-81

[7] PHOTOINDUCED ELECTRON-TRANSFER IN DNA MATRIX FROM INTERCALATED ETHIDIUM TO CONDENSED METHYLVIOLOGEN [J].

FROMHERZ, P ;

RIEGER, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (17) :5361-5362

[8] Oxidative DNA damage through long-range electron transfer [J].

Hall, DB ;

Holmlin, RE ;

Barton, JK .

NATURE, 1996, 382 (6593) :731-735

[9]

KERR JFR, 1994, CANCER-AM CANCER SOC, V73, P2013, DOI 10.1002/1097-0142(19940415)73:8<2013::AID-CNCR2820730802>3.0.CO

[10]

2-J

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