Perivenous expression of the mRNA of the three hypoxia-inducible factor α-subunits, HIF1α, HIF2α and HIF3α, in rat liver

被引：115

作者：

Kietzmann, T ^{[1
]}

Cornesse, Y ^{[1
]}

Brechtel, K ^{[1
]}

Modaressi, S ^{[1
]}

Jungermann, K ^{[1
]}

机构：

[1] Inst Biochem & Mol Zellbiol, D-37073 Gottingen, Germany

来源：

BIOCHEMICAL JOURNAL | 2001年 / 354卷

关键词：

glycolysis; hepatocyte; metabolic zonation; oxygen;

D O I：

10.1042/0264-6021:3540531

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNAs of three hypoxia-inducible factor (HIF) alpha -subunits were cloned from RNA of primary rat hepatocytes by reverse transcriptase PCR. All three cDNAs encoded functionally active proteins, of 825, 874 and 662 amino acids. After transfection they were able to activate luciferase activity of a luciferase gene construct containing three HIF-responsive elements. The mRNAs of the rat HIF alpha -subunits were expressed predominantly in the perivenous zone of rat liver tissue; the nuclear HIF alpha proteins, however, did not appear to be zonated.

引用

页码：531 / 537

页数：7

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