Dlxin-1, a novel protein that binds D1x5 and regulates its transcriptional function

被引：86

作者：

Masuda, Y

Sasaki, A

Shibuya, H

Ueno, N

Ikeda, K

Watanabe, K

机构：

[1] Natl Inst Longev Sci, Dept Geriatr Res, Aichi 4748522, Japan

[2] Natl Inst Basic Biol, Div Morphogenesis, Aichi 4448585, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 07期

关键词：

D O I：

10.1074/jbc.M008590200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dlx5, a member of the Dir family of homeodomain proteins, plays a critical role in bone development and fracture healing, To understand the molecular mechanism underlying the transcriptional regulation by Dlx5, we performed yeast two-hybrid screening and isolated a novel protein, Dlxin-1, that binds Dlx5 and regulates its transcriptional function. Dlxin-1 cDNA encodes a 775-amino acid protein that has a partial homology with necdin at the C terminus and 25 repeats of hexapeptides (WQXPXX) in the middle region. Dlxin-1 mRNA is expressed in various adult tissues, but not the spleen, and also in osteoblastic and chondrogenic cell lines. During embryogenesis, a strong signal for Dlxin-1 mRNA was found in cell layers surrounding cartilaginous elements in bone rudiment during dig-it formation. Dlxin-1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homo-multimers in vivo. Transfection and reporter gene assays indicate that Dlxin-1 activates the transcriptional function of Dlx5. Therefore, Dlxin-1 may act as a regulator of the function of Dir family members in bone formation.

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页码：5331 / 5338

页数：8

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