Considerations on antibody-phage display methodology

被引：27

作者：

Conrad, U

Scheller, J

机构：

[1] Univ Kiel, Inst Biochem, D-24098 Kiel, Germany

[2] Leibniz Inst, Inst Plant Genet & Crop Plant Res, IPK, D-06466 Gatersleben, Germany

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2005年 / 8卷 / 02期

关键词：

ScFv; M13; phage display; affinity selection; library; antigen;

D O I：

10.2174/1386207053258532

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

For almost 15 years phage display is being used for the selection of specific antigen-binders from artificial libraries of single chain antibodies. Filamentous phages have been developed in a way to express foreign proteins on the surface and at the same time carrying the genetic information of the surface expressed molecule within the phage capsid. This property guarantees the coupling of phenotype and genotype during phage amplification and affinity selection. The possibility to generate large antibody libraries and the simplified antibody-backbone of a single chain antibody has made antibody-phage display to a powerful tool for the development of new therapeutics against various human diseases. In this review we discuss the general principles and latest developments and applications in antibody phage display technology.

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收藏

页码：117 / 126

页数：10

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