High TNT-transforming activity by a mixed culture acclimated and maintained on crude-oll-containing media

被引：10

作者：

Popesku, JT

Singh, A

Zhao, JS

Hawari, J

Ward, OP ^{[1
]}

机构：

[1] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada

[2] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2003年 / 49卷 / 05期

关键词：

2,4,6-trinitrotoluene; mixed culture; transformation; co-metabolism; surfactant;

D O I：

10.1139/W03-049

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mixed microbial culture originating from a petroleum-contaminated site and maintained on crude oil exhibited high 2,4,6-trinitrotoluene (TNT) transformation activity. Cultivation of the mixed culture in glucose-containing medium for 29 h resulted in almost complete transformation of 100 ppm TNT. TNT transformation was observed with both growing and resting cells. With subculturing, it was found that TNT could support growth of the mixed culture when supplied as sole carbon source, sole nitrogen source, or sole carbon and nitrogen source. The finding that a mixed microbial culture maintained on crude oil exhibited high TNT transformation activity without prior subculture on TNT-containing media is novel and may have potential practical applications in the bioremediation of munitions-contaminated soil and wastewater.

引用

页码：362 / 366

页数：5

共 20 条

[1] PSEUDOMONAS-AERUGINOSA STRAIN MAO1 AEROBICALLY METABOLIZES THE AMINODINITROTOLUENES PRODUCED BY 2,4,6-TRINITROTOLUENE NITRO-GROUP REDUCTION [J].