Integration site for streptomyces phage φBT1 and development of site-specific integrating vectors

被引：229

作者：

Gregory, MA ^{[1
]}

Till, R ^{[1
]}

Smith, MCM ^{[1
]}

机构：

[1] Univ Nottingham, Queens Med Ctr, Inst Genet, Nottingham NG7 2UH, England

来源：

JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 17期

关键词：

D O I：

10.1128/JB.185.17.5320-5323.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Despite extensive similarities between the genomes of the Streptomyces temperate phages phiC31 and phiBT1, the attP-int loci are poorly conserved. Here we demonstrate that phiBT1 integrates into a different attachment site than phiC31. phiBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on phiBT1 integrase were shown to have a broad host range and are fully compatible with those based on the phiC31 attP-int locus.

引用

页码：5320 / 5323

页数：4

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