Development of a lesion-mimic phenotype in a transgenic wheat line overexpressing genes for pathogenesis-related (PR) proteins is dependent on salicylic acid concentration

被引:37
作者
Anand, A
Schmelz, EA
Muthukrishnan, S [1 ]
机构
[1] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA
[2] USDA ARS, CMAVE Chem, Gainesville, FL 32608 USA
关键词
programmed cell death;
D O I
10.1094/MPMI.2003.16.10.916
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the course of coexpressing genes for pathogenesis-related (PR) proteins for a class IV chitinase and an acidic glucanase in transgenic wheat plants, we regenerated a wheat line that developed necrotic lesions containing dead cells in the T-2 and subsequent generations. Lesion spots were detected at the booting stage (5- to 6-week-old plants) in lines homozygous for the transgene loci. In contrast, lesions were not observed in hemizygous transgenic lines or lines silenced for transgene expression, indicating a requirement for high levels of transgene expression for the development of the lesioned phenotype. Lesion development was associated with the accumulation of host-encoded PR proteins, e.g., chitinases, beta-1,3-glucanases, thaumatin-like protein, and production of reactive oxygen intermediates. F-1 progeny of a cross between the lesion-plus transgenic line and wild-type nontransgenic plants produced progeny with a normal phenotype, while the F-2 progenies segregated for the lesion phenotype. Salicylic acid (SA) levels in plants with the lesion-plus phenotype were found to be several times higher than controls and nearly double the levels in hemizygous transgenic plants that lack lesions. SA application activated lesion development in excised leaf pieces of these hemizygous transgenic plants. Similar activation of lesion development in control plants occurred only when high concentrations of SA were applied for prolonged periods. Transcripts for phenylalanine-ammonia lyase, which provides precursors of SA, were elevated in homozygous transgenic plants. Our data suggest that transgene-induced lesion-mimic phenotype correlates with enhanced SA biosynthesis.
引用
收藏
页码:916 / 925
页数:10
相关论文
共 63 条
[1]   INCREASED TOLERANCE TO 2 OOMYCETE PATHOGENS IN TRANSGENIC TOBACCO EXPRESSING PATHOGENESIS-RELATED PROTEIN-1A [J].
ALEXANDER, D ;
GOODMAN, RM ;
GUTRELLA, M ;
GLASCOCK, C ;
WEYMANN, K ;
FRIEDRICH, L ;
MADDOX, D ;
AHLGOY, P ;
LUNTZ, T ;
WARD, E ;
RYALS, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (15) :7327-7331
[2]   Stable transgene expression and random gene silencing in wheat [J].
Anand, A ;
Trick, HN ;
Gill, BS ;
Muthukrishnan, S .
PLANT BIOTECHNOLOGY JOURNAL, 2003, 1 (04) :241-251
[3]   Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum [J].
Anand, A ;
Zhou, T ;
Trick, HN ;
Gill, BS ;
Bockus, WW ;
Muthukrishnan, S .
JOURNAL OF EXPERIMENTAL BOTANY, 2003, 54 (384) :1101-1111
[4]   Genetic engineering of wheat for increased resistance to powdery mildew disease [J].
Bliffeld, M ;
Mundy, J ;
Potrykus, I ;
Fütterer, J .
THEORETICAL AND APPLIED GENETICS, 1999, 98 (6-7) :1079-1086
[5]  
BOWLES DJ, 1990, ANNU REV BIOCHEM, V59, P873, DOI 10.1146/annurev.bi.59.070190.004301
[6]   A MUTATION IN ARABIDOPSIS THAT LEADS TO CONSTITUTIVE EXPRESSION OF SYSTEMIC ACQUIRED-RESISTANCE [J].
BOWLING, SA ;
GUO, A ;
CAO, H ;
GORDON, AS ;
KLESSIG, DF ;
DONG, XI .
PLANT CELL, 1994, 6 (12) :1845-1857
[7]   TRANSGENIC PLANTS WITH ENHANCED RESISTANCE TO THE FUNGAL PATHOGEN RHIZOCTONIA-SOLANI [J].
BROGLIE, K ;
CHET, I ;
HOLLIDAY, M ;
CRESSMAN, R ;
BIDDLE, P ;
KNOWLTON, S ;
MAUVAIS, CJ ;
BROGLIE, R .
SCIENCE, 1991, 254 (5035) :1194-1197
[8]   Roles of salicylic acid, jasmonic acid, and ethylene in cpr-induced resistance in Arabidopsis [J].
Clarke, JD ;
Volko, SM ;
Ledford, H ;
Ausubel, FM ;
Dong, XN .
PLANT CELL, 2000, 12 (11) :2175-2190
[9]   Biosynthesis and action of jasmonates in plants [J].
Creelman, RA ;
Mullet, JE .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1997, 48 :355-381
[10]  
Dangl JL, 1996, PLANT CELL, V8, P1793, DOI 10.1105/tpc.8.10.1793