Promoter trapping identifies real genes in C-elegans

被引：14

作者：

Hope, IA ^{[1
]}

Arnold, JM ^{[1
]}

McCarroll, D ^{[1
]}

Jun, G ^{[1
]}

Krupa, AP ^{[1
]}

Herbert, R ^{[1
]}

机构：

[1] Univ Leeds, Sch Biol, Leeds LS2 9JT, W Yorkshire, England

来源：

MOLECULAR AND GENERAL GENETICS | 1998年 / 260卷 / 2-3期

基金：

英国医学研究理事会;

关键词：

Caenorhabditis elegans; gene expression patterns; genome sequence;

D O I：

10.1007/s004380050899

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Promoter trapping involved screening uncharacterized fragments of C. elegans genomic DNA for C. elegans promoter activity. By sequencing the ends of these DNA fragments and locating their genomic origin using the available genome sequence data, promoter trapping has now been shown to identify real promoters of real genes, exactly as anticipated. Developmental expression patterns have thereby been linked to gene sequence, allowing further inferences on gene function to be drawn. Some expression patterns generated by promoter trapping include subcellular details. Localization to the surface of particular cells or even particular aspects of the cell surface was found to be consistent with the genes, now associated with these patterns, encoding membrane-spanning proteins. Data on gene expression patterns are easier to generate and characterize than mutant phenotypes and may provide the best means of interpreting the large quantity of sequence data currently being generated in genome projects.

引用

页码：300 / 308

页数：9

共 28 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2] CIS AND TRANS MESSENGER-RNA SPLICING IN C-ELEGANS [J].

BLUMENTHAL, T ;

THOMAS, J .

TRENDS IN GENETICS, 1988, 4 (11) :305-308

[3]

Brill S, 1996, MOL CELL BIOL, V16, P4869

[4]

COOPER AF, 1971, J NEMATOL, V3, P205

[5] TOWARD A PHYSICAL MAP OF THE GENOME OF THE NEMATODE CAENORHABDITIS-ELEGANS [J].

COULSON, A ;

SULSTON, J ;

BRENNER, S ;

KARN, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (20) :7821-7825

[6] GENOME LINKING WITH YEAST ARTIFICIAL CHROMOSOMES [J].

COULSON, A ;

WATERSTON, R ;

KIFF, J ;

SULSTON, J ;

KOHARA, Y .

NATURE, 1988, 335 (6186) :184-186

[7]

EECKMAN FH, 1995, METHOD CELL BIOL, V48, P584

[8] INFORMATION-CONTENT OF CAENORHABDITIS-ELEGANS SPLICE SITE SEQUENCES VARIES WITH INTRON LENGTH [J].

FIELDS, C .

NUCLEIC ACIDS RESEARCH, 1990, 18 (06) :1509-1512

[9] A MODULAR SET OF LACZ FUSION VECTORS FOR STUDYING GENE-EXPRESSION IN CAENORHABDITIS-ELEGANS [J].

FIRE, A ;

HARRISON, SW ;

DIXON, D .

GENE, 1990, 93 (02) :189-198

[10] MOLECULAR CHARACTERIZATION OF A MEMBRANE TRANSPORTER FOR LACTATE, PYRUVATE, AND OTHER MONOCARBOXYLATES - IMPLICATIONS FOR THE CORI CYCLE [J].

GARCIA, CK ;

GOLDSTEIN, JL ;

PATHAK, RK ;

ANDERSON, RGW ;

BROWN, MS .

CELL, 1994, 76 (05) :865-873

← 1 2 3 →