Synthesis and characterization of a polymer-coated C18 stationary phase with high carbon content for liquid chromatography

被引：30

作者：

Kobayashi, S ^{[1
]}

Tanaka, I ^{[1
]}

Shirota, O ^{[1
]}

Kanda, T ^{[1
]}

Ohtsu, Y ^{[1
]}

机构：

[1] Shiseido Res Ctr, Kohoku Ku, Yokohama, Kanagawa 2238553, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 828卷 / 1-2期

关键词：

stationary phases; LC; polymer coatings; pyridine; phenol; hinokitiol;

D O I：

10.1016/S0021-9673(98)00686-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-purity porous silica with a large surface area (particle diameter 5 mu m, mean pore diameter 8.4 nm, surface area 350 m(2)/g) was coated with a homogeneous silicone polymer film, which was thereafter modified with octadecyl groups to be used as a stationary phase for reversed-phase chromatography. The stationary phase had a high carbon content (18%), and showed a relatively large overall retention for various analytes among other stationary phases available in the market. It also showed preferable characteristics in separation of basic compounds, such as pyridine, and hinokitiol, a substance that easily coordinates with metals, and also had a high durability under an alkaline mobile phase. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：75 / 81

页数：7

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