The rice mitochondrial iron transporter is essential for plant growth

被引：130

作者：

Bashir, Khurram ^{[1
]}

Ishimaru, Yasuhiro ^{[1
]}

Shimo, Hugo ^{[1
]}

Nagasaka, Seiji ^{[2
]}

Fujimoto, Masaru ^{[1
]}

Takanashi, Hideki ^{[1
]}

Tsutsumi, Nobuhiro ^{[1
]}

An, Gynheung ^{[3
]}

Nakanishi, Hiromi ^{[1
]}

Nishizawa, Naoko K. ^{[1
,4
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[2] Toyo Univ, Grad Sch Life Sci, Gunma 3740193, Japan

[3] Kyung Hee Univ, Biotech & Crop Biotech Inst, Dept Plant Mol Syst, Yongin 446701, South Korea

[4] Ishikawa Prefectural Univ, Res Inst Bioresources & Biotechnol, Nonoichi, Ishikawa 9218836, Japan

来源：

NATURE COMMUNICATIONS | 2011年 / 2卷

关键词：

EFFICIENT TRANSFORMATION; SIGNALING PATHWAY; ORYZA-SATIVA; T-DNA; ARABIDOPSIS; YEAST; PROTEINS; MITOFERRIN; MEMBRANE; CLONING;

D O I：

10.1038/ncomms1326

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In plants, iron (Fe) is essential for mitochondrial electron transport, heme, and Fe-Sulphur (Fe-S) cluster synthesis; however, plant mitochondrial Fe transporters have not been identified. Here we show, identify and characterize the rice mitochondrial Fe transporter (MIT). Based on a transfer DNA library screen, we identified a rice line showing symptoms of Fe deficiency while accumulating high shoot levels of Fe. Homozygous knockout of MIT in this line resulted in a lethal phenotype. MIT localized to the mitochondria and complemented the growth of Delta mrs3 Delta mrs4 yeast defective in mitochondrial Fe transport. The growth of MIT-knockdown (mit-2) plants was also significantly impaired despite abundant Fe accumulation. Further, the decrease in the activity of the mitochondrial and cytosolic Fe-S enzyme, aconitase, indicated that Fe-S cluster synthesis is affected in mit-2 plants. These results indicate that MIT is a mitochondrial Fe transporter essential for rice growth and development.

引用

页数：7

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