Phosphorylated modification and in vitro antioxidant activity of Radix Hedysari polysaccharide

被引：65

作者：

Wei, Dongfeng ^{[1
]}

Cheng, Weidong ^{[1
,2
]}

Wei, Yanxia ^{[1
]}

Zhang, Lifeng ^{[1
]}

机构：

[1] Lanzhou Univ, Sch Basic Med Sci, Lanzhou 730000, Peoples R China

[2] Beijing Normal Univ, Coll Resources Sci & Technol, Beijing 100009, Peoples R China

来源：

GLYCOCONJUGATE JOURNAL | 2012年 / 29卷 / 04期

基金：

中国博士后科学基金;

关键词：

Radix Hedysari; Polysaccharide; Phosphorylated modification; Antioxidant activity; OXIDATIVE STRESS; DERIVATIVES; ASTRAGALI;

D O I：

10.1007/s10719-012-9377-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Phosphorylated modification of a polysaccharide obtained from Radix Hedysari (RHP) was studied. Three phosphorylated polysaccharides (RHPP) with variable degrees of substitution (DSp) were obtained with 4-dimethylaminopyridine (DMAP) and N, N' Dicyclocarbodiimide (DCC) as catalyst. The structures of RHPP were characterized by FT-IR spectra and C-13 NMR spectra. Depending on different reaction time, RHPP showed different DSp ranging from 0.30 to 0.66, and different Mw ranging from 86.6 to 89.7 KDa. Compared with RHP, RHPP exhibited superior antioxidant activities in vitro, which indicated that phosphorylated modification could enhance antioxidant activities of RHP. Furthermore, it was obvious that the DSp had a significant effect on the antioxidant activity.

引用

页码：167 / 172

页数：6

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