Horizontal transfer of segments of the 16S rRNA genes between species of the Streptococcus anginosus group

被引:69
作者
Schouls, LM
Schot, CS
Jacobs, JA
机构
[1] Natl Inst Publ Hlth & Environm, Lab Vaccine Preventable Dis, NL-3720 BA Bilthoven, Netherlands
[2] Univ Hosp Maastricht, Dept Med Microbiol, Maastricht, Netherlands
关键词
D O I
10.1128/JB.185.24.7241-7246.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The nature in variation of the 16S rRNA gene of members of the Streptococcus anginosus group was investigated by hybridization and DNA sequencing. A collection of 708 strains was analyzed by reverse line blot hybridization. This revealed the presence of distinct reaction patterns representing 11 different hybridization groups. The 16S rRNA genes of two strains of each hybridization group were sequenced to near-completion, and the sequence data confirmed the reverse line blot hybridization results. Closer inspection of the sequences revealed mosaic-like structures, strongly suggesting horizontal transfer of segments of the 16S rRNA gene between different species belonging to the Streptococcus anginosus group. Southern blot hybridization further showed that within a single strain all copies of the 16S rRNA gene had the same composition, indicating that the apparent mosaic structures were not PCR-induced artifacts. These findings indicate that the highly conserved rRNA genes are also subject to recombination and that these events may be fixed in the population. Such recombination may lead to the construction of incorrect phylogenetic trees based on the 16S rRNA genes.
引用
收藏
页码:7241 / 7246
页数:6
相关论文
共 26 条
[1]   An Escherichia coli strain with all chromosomal rRNA operons inactivated:: Complete exchange of rRNA genes between bacteria [J].
Asai, T ;
Zaporojets, D ;
Squires, C ;
Squires, CL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (05) :1971-1976
[2]   Corresponding 16S rRNA gene segments in Rhizobiaceae and Aeromonas yield discordant phylogenies [J].
Eardly, BD ;
Wang, FS ;
vanBerkum, P .
PLANT AND SOIL, 1996, 186 (01) :69-74
[3]  
GOSSLING J, 1988, REV INFECT DIS, V10, P257
[4]  
Goto T, 2002, J MED MICROBIOL, V51, P178, DOI 10.1099/0022-1317-51-2-178
[5]   Rates and consequences of recombination between rRNA operons [J].
Hashimoto, JQ ;
Stevenson, BS ;
Schmidt, TM .
JOURNAL OF BACTERIOLOGY, 2003, 185 (03) :966-972
[6]   Natural competence in the genus Streptococcus: Evidence that streptococci can change pherotype by interspecies recombinational exchanges [J].
Havarstein, LS ;
Hakenbeck, R ;
Gaustad, P .
JOURNAL OF BACTERIOLOGY, 1997, 179 (21) :6589-6594
[7]  
JACOB AE, 1989, MICROBIOS, V60, P167
[8]   Haemolytic activity of the 'Streptococcus milleri group' and relationship between haemolysis restricted to human red blood cells and pathogenicity in S-intermedius [J].
Jacobs, JA ;
Schot, CS ;
Schouls, LM .
JOURNAL OF MEDICAL MICROBIOLOGY, 2000, 49 (01) :55-62
[9]   The Streptococcus anginosus species comprises five 16S rRNA ribogroups with different phenotypic characteristics and clinical relevance [J].
Jacobs, JA ;
Schot, CS ;
Schouls, LM .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :1073-1079
[10]   DNA-DNA reassociation studies of Streptococcus constellatus with unusual 16S rRNA sequences [J].
Jacobs, JA ;
Schouls, LM ;
Whiley, RA .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :247-249