In vitro anti-oxidant activity, fluorescence quenching study and structural features of carbohydrate polymers from Phyllanthus emblica

被引：26

作者：

Chatterjee, Udipta Ranjan ^{[1
]}

Bandyopadhyay, Shruti S. ^{[1
]}

Ghosh, Debjani ^{[1
]}

Ghosal, Pradyot K. ^{[1
]}

Ray, Bimalendu ^{[1
]}

机构：

[1] Univ Burdwan, Nat Prod Lab, Dept Chem, Burdwan 713104, W Bengal, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2011年 / 49卷 / 04期

关键词：

Phyllanthus emblica; Carbohydrate polymers; Structural features; Anti-oxidant activity; Fluorescence quenching; POLYSACCHARIDES; POWER;

D O I：

10.1016/j.ijbiomac.2011.06.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The water-extracted carbohydrate polymers (WE) of Phyllanthus emblica are analyzed using chemical, chromatographic, and spectroscopic methods. Anion-exchange-chromatography of WE yielded four fractions (F1-F4) with different chemical compositions and all of them contain phenolics. The major fraction F4 possesses 50% polysaccharide and 26% phenol, and is a glycoconjugate. The antioxidant capacities of WE and F4 are comparable to standard anti-oxidants. Notably, activities of F1-F4 correlate with their phenol content. Evidence for the complexation of F4 with bovine serum albumin is presented by fluorescence quenching measurement. The results also indicate conformational change of protein at high carbohydrate polymer concentration. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：637 / 642

页数：6

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