Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4

被引:456
作者
Feys, BJ [1 ]
Moisan, LJ [1 ]
Newman, MA [1 ]
Parker, JE [1 ]
机构
[1] John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
关键词
Arabidopsis; dimerization; EDS1; PAD41; salicylic acid;
D O I
10.1093/emboj/20.19.5400
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Arabidopsis EDS1 and PAD4 genes encode lipase-like proteins that function in resistance (R) gene-mediated and basal plant disease resistance. Phenotypic analysis of eds] and pad4 null mutants shows that EDS1 and PAD4 are required for resistance conditioned by the same spectrum of R genes but fulfil distinct roles within the defence pathway. EDS1 is essential for elaboration of the plant hypersensitive response, whereas EDS1 and PAD4 are both required for accumulation of the plant defence-potentiating molecule, salicylic acid. EDS1 is necessary for pathogen-induced PAD4 mRNA accumulation, whereas mutations in PAD4 or depletion of salicylic acid only partially compromise EDS1 expression. Yeast two-hybrid analysis reveals that EDS1 can dimerize and interact with PAD4. However, EDS1 dimerization is mediated by different domains to those involved in EDS1-PAD4 association. Co-immunoprecipitation experiments show that EDS1 and PAD4 proteins interact in healthy and pathogen-challenged plant cells. We propose two functions for EDS1. The first is required early in plant defence, independently of PAD4. The second recruits PAD4 in the amplification of defences, possibly by direct EDS1-PAD4 association.
引用
收藏
页码:5400 / 5411
页数:12
相关论文
共 43 条
[1]   Different requirements for EDS1 and NDR1 by disease resistance genes define at least two R gene-mediated signaling pathways in Arabidopsis [J].
Aarts, N ;
Metz, M ;
Holub, E ;
Staskawicz, BJ ;
Daniels, MJ ;
Parker, JE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (17) :10306-10311
[2]   Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues [J].
An, YQ ;
McDowell, JM ;
Huang, SR ;
McKinney, EC ;
Chambliss, S ;
Meagher, RB .
PLANT JOURNAL, 1996, 10 (01) :107-121
[3]  
Ausubel F.M., 1996, CURRENT PROTOCOLS MO
[4]   Forms of lipoprotein lipase in rat tissues: In adipose tissue the proportion of inactive lipase increases on fasting [J].
Bergo, M ;
Olivecrona, G ;
Olivecrona, T .
BIOCHEMICAL JOURNAL, 1996, 313 :893-898
[5]   Three genes of the arabidopsis RPP1 complex resistance locus recognize distinct Peronospora parasitica avirulence determinants [J].
Botella, MA ;
Parker, JE ;
Frost, LN ;
Bittner-Eddy, PD ;
Beynon, JL ;
Daniels, MJ ;
Holub, EB ;
Jones, JDG .
PLANT CELL, 1998, 10 (11) :1847-1860
[6]   The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance [J].
Bowling, SA ;
Clarke, JD ;
Liu, YD ;
Klessig, DF ;
Dong, XN .
PLANT CELL, 1997, 9 (09) :1573-1584
[7]   Constitutive disease resistance requires EDS1 in the Arabidopsis mutants cpr1 and cpr6 and is partially EDS1-dependent in cpr5 [J].
Clarke, JD ;
Aarts, N ;
Feys, BJ ;
Dong, XN ;
Parker, JE .
PLANT JOURNAL, 2001, 26 (04) :409-420
[8]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743
[9]   Nitric oxide functions as a signal in plant disease resistance [J].
Delledonne, M ;
Xia, YJ ;
Dixon, RA ;
Lamb, C .
NATURE, 1998, 394 (6693) :585-588
[10]   Salicylic acid and disease resistance in plants [J].
Dempsey, DA ;
Shah, J ;
Klessig, DF .
CRITICAL REVIEWS IN PLANT SCIENCES, 1999, 18 (04) :547-575