Proteomic analysis of rat microglia establishes a high-confidence reference data set of over 3000 proteins

被引：14

作者：

Bell-Temin, Harris ^{[1
]}

Barber, David S. ^{[2
]}

Zhang, Ping ^{[3
]}

Liu, Bin ^{[3
]}

Stevens, Stanley M., Jr. ^{[1
]}

机构：

[1] Univ S Florida, Dept Cell Biol Microbiol & Mol Biol, Tampa, FL 33620 USA

[2] Univ Florida, Dept Physiol Sci, Ctr Environm & Human Toxicol, Gainesville, FL 32610 USA

[3] Univ Florida, Coll Pharm, Dept Pharmacodynam, Gainesville, FL 32610 USA

来源：

PROTEOMICS | 2012年 / 12卷 / 02期

关键词：

Cell biology; Glia; LC-MS; Microglia; Protein profile; OLIGODENDROCYTES; ASTROCYTES; RELEASE; PRIDE;

D O I：

10.1002/pmic.201100398

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Highly aggressively proliferating immortalized (HAPI) microglial cells have been used as an in vitro model for investigating key microglial functions including inflammatory, neurotoxic, and phagocytic activities. Through the use of offline strong cation-exchange fractionation followed by inline reversed-phase chromatographic separation and tandem mass spectrometric analysis on a hybrid linear ion trap-Orbitrap instrument, the HAPI microglial proteome was characterized to a depth of 3006 unique protein groups. Upon bioinformatic analysis of the HAPI proteome data set, enrichment was observed for processes relevant to microglial function including those associated with immune system response. This study marks the most comprehensive reference data set generated to date for the rat microglial proteome.

引用

页码：246 / 250

页数：5

共 14 条

[1]

Aloisi F, 1999, ADV EXP MED BIOL, V468, P123

[2]

Barsnes H, 2011, METHODS MOL BIOL, V694, P237, DOI 10.1007/978-1-60761-977-2_16

[3]

Characterization of a novel brain-derived microglial cell line isolated from neonatal rat brain [J].

Cheepsunthorn, P ;

Radov, L ;

Menzies, S ;

Reid, J ;

Connor, JR .

GLIA, 2001, 35 (01) :53-62

[4]

Cathepsin S Release from Primary Cultured Microglia Is Regulated by the P2X7 Receptor [J].

Clark, Anna K. ;

Wodarski, Rachel ;

Guida, Francesca ;

Sasso, Oscar ;

Malcangio, Marzia .

GLIA, 2010, 58 (14) :1710-1726

[5]

Spinal upregulation of the nitric oxide synthase-interacting protein NOSIP in a rat model of inflammatory pain [J].

Dreyer, J ;

Hirlinger, D ;

Müller-Esterl, W ;

Oess, S ;

Kuner, R .

NEUROSCIENCE LETTERS, 2003, 350 (01) :13-16

[6]

Role of microglia in inflammation-mediated neurodegenerative diseases: Mechanisms and strategies for therapeutic intervention [J].

Liu, B ;

Hong, JS .

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2003, 304 (01) :1-7

[7]

Modulation of microglial pro-inflammatory and neurotoxic activity for the treatment of Parkinson's disease [J].

Liu, Bin .

AAPS JOURNAL, 2006, 8 (03) :E606-E621

[8]

PRIDE: The proteomics identifications database [J].

Martens, L ;

Hermjakob, H ;

Jones, P ;

Adamski, M ;

Taylor, C ;

States, D ;

Gevaert, K ;

Vandekerckhove, J ;

Apweiler, R .

PROTEOMICS, 2005, 5 (13) :3537-3545

[9]

EFFECTS OF INTERLEUKIN-1 AND TUMOR-NECROSIS-FACTOR-ALPHA ON ASTROCYTES, MICROGLIA, OLIGODENDROCYTES, AND GLIAL PRECURSORS INVITRO [J].

MERRILL, JE .

DEVELOPMENTAL NEUROSCIENCE, 1991, 13 (03) :130-&

[10]

NATURAL AND INDUCED CYTOTOXICITY OF OLIGODENDROCYTES BY MICROGLIA IS INHIBITABLE BY TGF-BETA [J].

MERRILL, JE ;

ZIMMERMAN, RP .

GLIA, 1991, 4 (03) :327-331

← 1 2 →