Behavioral and morphologic studies of the chronically compressed cauda equina - Experimental model of lumbar spinal stenosis in the rat

Study Design. An experimental model in rats of chronically compressed cauda equina was produced, and behavioral and morphologic changes were examined. Objectives. To provide a useful model for analyzing the pathophysiologic changes of the cauda equina by chronic compression and to examine behavioral and morphologic changes in this model. Summary of Background Data. Several animal models have been reported in which various materials were used to compress the cauda equina, However, the pathophysiology of the cauda equina by chronic compression is not yet well understood. Studies in which rats were used are scarce. Methods. A silicone sheet was applied to the spinal canal at L4 in the rat. Walking durations on treadmill tests and paw-withdrawal latencies to thermal stimuli were measured before and after the operation for 24 weeks. Histologic changes also were examined. Results. Walking durations decreased after chronic compression. However, paw-withdrawal latencies were not significantly changed. Histologically, the number of large-diameter myelinated axons decreased after compression, whereas the number of small-diameter myelinated axons increased. Electron microscopic observation indicated that the continuous degeneration and regeneration of axons occurred throughout the chronic compression experiment. Conclusions. The current model and behavioral assessments may be useful in analyzing the pathophysiology of chronically compressed cauda equina.