Determination of galacturonic acid from pomelo pectin in term of galactose by HPAEC with fluorescence detection

被引：61

作者：

Burana-osot, Jankana ^{[2
]}

Soonthornchareonnon, Noppamas ^{[3
]}

Chaidedgumjorn, Amornrut ^{[2
]}

Hosoyama, Saori ^{[1
]}

Toida, Toshihiko ^{[1
]}

机构：

[1] Chiba Univ, Grad Sch Pharmaceut Sci, Chiba 2638522, Japan

[2] Silpakorn Univ, Fac Pharm, Dept Pharmaceut Chem, Nakhon Pathom 73000, Thailand

[3] Mahidol Univ, Fac Pharm, Dept Pharmacognosy, Bangkok 10400, Thailand

来源：

CARBOHYDRATE POLYMERS | 2010年 / 81卷 / 02期

基金：

日本学术振兴会;

关键词：

Galacturonic acid; Galactose; Pectin; HPAEC-fluorescence detector; PERFORMANCE LIQUID-CHROMATOGRAPHY; POLYSACCHARIDES; PLASMA; ROOTS;

D O I：

10.1016/j.carbpol.2010.03.001

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Galacturonic acid (GalA) in pomelo pectin was converted to galactose (Gal) by saponification and reduction reaction of carboxy group. The characterization of the product was investigated by H-1 NMR spectroscopy. The reduced pectin was then hydrolyzed with trifluoroacetic acid (TFA) and analyzed by high-performance anion-exchange chromatography (HPAEC) with fluorescence detector. The content of GalA was calculated from an increased Gal after reaction. The GalA content obtained from this method was higher than that obtained from HPAEC-pulsed amperometric detection (PAD), which affords to analyze GalA directly after hydrolysis. The established method was validated and the results showed good linearity, high precision and high sensitivity. This method could be further applied for the analysis of GalA content in pomelo pectin. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：461 / 465

页数：5

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