Ribosomal DNA fragments enhance the stability of transfected DNA in Entamoeba histolytica

被引：6

作者：

Dhar, SK

Vines, RR

Bhattacharya, S

Petri, WA ^{[1
]}

机构：

[1] Univ Virginia, Dept Med, Charlottesville, VA 22908 USA

[2] Univ Virginia, Dept Microbiol & Pathol, Charlottesville, VA 22908 USA

[3] Jawaharlal Nehru Univ, Sch Environm Sci, New Delhi 110067, India

来源：

JOURNAL OF EUKARYOTIC MICROBIOLOGY | 1998年 / 45卷 / 06期

关键词：

rDNA circle; replication; stable transfection;

D O I：

10.1111/j.1550-7408.1998.tb04563.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Genetic manipulation of Entamoeba histolytica is limited by the inability to express foreign genes at high levels. We tested whether sequences from the E. histolytica rDNA episome, present in 200 copies per cell, could act to stabilize the episomal transfection vector pTCV1. Ligation of the rDNA transcription unit, or sequences downstream of the rDNA transcription unit, increased pTCV1 copy number and stability and conferred additional zones of DNA replication. Sequences upstream of the rDNA transcription unit dramatically destabilized pTCV1. These experiments give additional insights into the mechanism of DNA replication and provide for E. histolytica a set of transfection vectors with unique properties.

引用

页码：656 / 660

页数：5

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