Nonaqueous capillary electrophoretic separation and thermo-optical absorbance detection of five tricyclic antidepressants and metabolism of amitriptyline by Cunninghamella elegans

被引:23
作者
Li, XF
Liu, CS
Roos, P
Hansen, EB
Cerniglia, CE
Dovichi, NJ [1 ]
机构
[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada
[2] Sciex, MDS Hlth Grp, Concord, ON, Canada
[3] US FDA, Natl Ctr Toxicol Res, Div Microbiol & Chem, Jefferson, AR 72079 USA
关键词
antidepressants; nonaqueous capillary electrophoresis; thermo-optical absorbance;
D O I
10.1002/elps.1150191821
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We developed a technique based on nonaqueous capillary electrophoresis and laser-based thermo-optical absorbance detection to assay five antidepressants with similar structures and mass-to-charge ratios. A mixture of methanol and acetonitrile with ammonium acetate was essential to achieve baseline resolution of these compounds. We investigated the effects of ammonium acetate concentration, temperature, applied voltage, and capillary length on separation efficiency. The nonaqueous capillary electrophoresis and laser-based thermo-optical absorbance detection technique was used to study the metabolism of amitriptyline by Cunninghamella elegans. Sample preparation procedures were simplified for fast screening of the parent drug and its metabolites. Reproducible electropherograms were obtained from replicate cultures of C. elegans growing in the presence of amitriptyline.
引用
收藏
页码:3178 / 3182
页数:5
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