Synchronous luminescence: a simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein

被引：3

作者：

Askari, M

Miller, G

Vo-Dinh, T ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Div Life Sci, Adv Monitoring Dev Grp, Oak Ridge, TN 37831 USA

[2] Univ Tennessee, Grad Sch Biomed Sci, Oak Ridge, TN 37831 USA

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 20期

关键词：

fragile histidine triad gene; fluorescence spectroscopy; hydrolysis; synchronous luminescence spectroscopy;

D O I：

10.1023/A:1012404430463

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Human fragile histidine triad (FHIT) protein has dinucleoside 5',5'''-P-1,P-n-polyphosphates hydrolysis activity, with AMP being one of the reaction products. Application of synchronous luminescence (SL) spectroscopy, in which both excitation and emission wavelengths are scanned simultaneously while a constant wavelength interval is maintained between them, was investigated for detection of the enzymatic activity of the FHIT protein. Ability of SL to identify reaction components, AMP production and its increase as a result of increase in substrate concentration and inhibition of the hydrolysis activity by ZnCl2 are demonstrated.

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页码：1697 / 1702

页数：6

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