Detection and survival of pathogens during two-stage thermophilic/mesophilic anaerobic treatment of suspended organic waste

被引：5

作者：

Burtscher, C ^{[1
]}

Fall, PA ^{[1
]}

Christ, O ^{[1
]}

Wilderer, PA ^{[1
]}

Wuertz, S ^{[1
]}

机构：

[1] Tech Univ Munich, Inst Water Qual Control & Waste Management, D-85748 Garching, Germany

来源：

WATER SCIENCE AND TECHNOLOGY | 1998年 / 38卷 / 12期

关键词：

anaerobic digestion; hygienic performance; nucleic acid extraction; organic waste; pathogens; polymerase chain reaction;

D O I：

10.1016/S0273-1223(98)00812-9

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The food-borne pathogens Salmonella typhimurium and Listeria monocytogenes were effectively eliminated from suspended organic waste during the thermophilic (55 degrees C) phase of a two-stage thermophilic/mesophilic anaerobic reactor. The feasibility of PCR technology was evaluated in order to improve the speed of detection and reduce the effort involved in detecting the presence of a range of pathogenic bacteria. Our results show direct extraction of nucleic acids followed by purification and PCR to be a promising method that will enable the rapid verification of hygienic performance during waste treatment. (C) 1998 IAWQ. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：123 / 126

页数：4

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