Systems analysis of adaptive immunity by utilization of high-throughput technologies

被引：21

作者：

Reddy, Sai T. ^{[1
,2
]}

Georgiou, George ^{[1
,2
,3
,4
]}

机构：

[1] Univ Texas Austin, Dept Chem Engn, Austin, TX 78712 USA

[2] Univ Texas Austin, Dept Biomed Engn, Austin, TX 78712 USA

[3] Univ Texas Austin, Inst Cellular & Mol Biol, Austin, TX 78712 USA

[4] Univ Texas Austin, Sect Mol Genet & Microbiol, Austin, TX 78712 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2011年 / 22卷 / 04期

关键词：

HUMAN MONOCLONAL-ANTIBODIES; B-CELLS; RESPONSES; MICROARRAYS; EXPRESSION; GENERATION; VACCINE; PROTEIN; REPERTOIRES; DIVERSITY;

D O I：

10.1016/j.copbio.2011.04.015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new generation of high-throughput technologies for quantitative and clonal analysis of adaptive immune responses have been developed. Functional analysis of lymphocyte populations has been accomplished via microfluidic assay systems. Additionally, lymphocyte receptor repertoires have been characterized on proteomic and genomic levels with multiplexed protein microarrays and high-throughput DNA sequencing. These tools are providing an unprecedented level of information depth on the distribution of adaptive immune cell (B and T cell) functionalities and repertoires, which develop upon activation following vaccination, pathogenic infection, or in disease states. These various high-throughput technologies have unlocked the potential to transform immunology into an information-rich science that will enable rapid expansion of the field of experimental systems immunology.

引用

页码：584 / 589

页数：6

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