In situ expression of two storage protein genes in relation to histo-differentiation at mid-embryogenesis in Medicago truncatula and Pisum sativum seeds

被引:14
作者
Abirached-Darmency, M
Abdel-gawwad, MR
Conejero, G
Verdeil, JL
Thompson, R
机构
[1] INRA, URLEG, Unite Rech Legumineuses, F-21065 Dijon, France
[2] CIRAD UMR BEPC, F-34198 Montpellier, France
关键词
in situ hybridization; legumin A mRNA; Medicago truncatula; mid-embryogenesis; Pisum sativum; seed; vicilin mRNA;
D O I
10.1093/jxb/eri200
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The seed consists of several layers of specialized cell-types that divide and differentiate following a highly regulated programme in time and space. A cytological approach was undertaken in order to study the histo-differentiation at mid-embryogenesis in Medicago truncatula as a model legume, and in Pisum sativum using serial sections of embedded immature seed. Little published information is available about seed development in Medicago species. The observations from this study revealed a number of distinctive features of Medicago seed development and differentiation. Transfer cells, involved in nutrient transfer to the embryo, were clearly identified in the thin-walled parenchyma of the innermost integument. Histological Schiff-naphthol enabled carbohydrate accumulation to be followed in the different seed compartments, and revealed the storage protein bodies. Non-radioactive mRNA in situ hybridization, was carried out using mRNA probes from two highly expressed genes encoding the major vicilin and legumin A storage protein types. The timing of mRNA expression was related to that of the corresponding proteins already identified.
引用
收藏
页码:2019 / 2028
页数:10
相关论文
共 38 条
[1]   Immunolocalization of carbonic anhydrase and phosphoenolpyruvate carboxylase in developing seeds of Medicago sativa [J].
Aivalakis, G ;
Dimou, M ;
Flemetakis, E ;
Plati, F ;
Katinakis, P ;
Drossopoulos, JB .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2004, 42 (03) :181-186
[2]  
[Anonymous], 1962, ANATOMY SEED PLANTS
[3]   An integrated overview of seed development in Arabidopsis thaliana ecotype WS [J].
Baud, S ;
Boutin, JP ;
Miquel, M ;
Lepiniec, L ;
Rochat, C .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2002, 40 (02) :151-160
[4]   Endosperm development [J].
Berger, F .
CURRENT OPINION IN PLANT BIOLOGY, 1999, 2 (01) :28-32
[5]   Embryogenesis of Vicia faba L: Histodifferentiation in relation to starch and storage protein synthesis [J].
Borisjuk, L ;
Weber, H ;
Panitz, R ;
Manteuffel, R ;
Wobus, U .
JOURNAL OF PLANT PHYSIOLOGY, 1995, 147 (02) :203-218
[6]   Spatial analysis of plant metabolism:: Sucrose imaging within Vicia faba cotyledons reveals specific developmental patterns [J].
Borisjuk, L ;
Walenta, S ;
Rolletschek, H ;
Mueller-Klieser, W ;
Wobus, U ;
Weber, H .
PLANT JOURNAL, 2002, 29 (04) :521-530
[7]  
Borisjuk L, 2002, DEVELOPMENT, V129, P1595
[8]  
Casey R, 2001, NAHRUNG, V45, P385, DOI 10.1002/1521-3803(20011001)45:6&lt
[9]  
385::AID-FOOD385&gt
[10]  
3.0.CO