Single nucleotide polymorphism genotyping: biochemistry, protocol, cost and throughput

被引：165

作者：

Chen, X ^{[1
]}

Sullivan, PF ^{[1
]}

机构：

[1] Virginia Commonwealth Univ, Virginia Inst Psychiat & Behav Genet, Dept Psychiat, Richmond, VA 23298 USA

来源：

PHARMACOGENOMICS JOURNAL | 2003年 / 3卷 / 02期

关键词：

SNP; genotyping; allele discrimination; cost; throughput;

D O I：

10.1038/sj.tpj.6500167

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The large number of single nucleotide polymorphism ( SNP) markers available in the public databases makes studies of association and fine mapping of disease loci very practical. To provide information for researchers who do not follow SNP genotyping technologies but need to use them for their research, we review here recent developments in the fields. We start with a general description of SNP typing protocols and follow this with a summary of current methods for each step of the protocol and point out the unique features and weaknesses of these techniques as well as comparing the cost and throughput structures of the technologies. Finally, we describe some popular techniques and the applications that are suitable for these techniques.

引用

页码：77 / 96

页数：20

共 136 条

[1] Abdi F, 2001, Nucleic Acids Res, V29, pE61, DOI 10.1093/nar/29.13.e61
[2] Comparison study for identifying promoter allelic polymorphism in interleukin 10 and tumor necrosis factor α genes
Agarwal, P
Oldenburg, MC
Czarneski, JE
Morse, RM
Hameed, MR
Cohen, S
Fernandes, H
[J]. DIAGNOSTIC MOLECULAR PATHOLOGY, 2000, 9 (03) : 158 - 164
[3] Utility and accuracy of template-directed dye-terminator incorporation with fluorescence-polarization detection for genotyping single nucleotide polymorphisms
Akula, N
Chen, YS
Hennessy, K
Schulze, TG
Singh, G
McMahon, FJ
[J]. BIOTECHNIQUES, 2002, 32 (05) : 1072 - +
[4] Armstrong B, 2000, CYTOMETRY, V40, P102, DOI 10.1002/(SICI)1097-0320(20000601)40:2<102::AID-CYTO3>3.3.CO
[5] 2-W
[6] Discrimination of primer 3′-nucleotide mismatch by Taq DNA polymerase during polymerase chain reaction
Ayyadevara, S
Thaden, JJ
Reis, RJS
[J]. ANALYTICAL BIOCHEMISTRY, 2000, 284 (01) : 11 - 18
[7] Association testing by DNA pooling: An effective initial screen
Bansal, A
van den Boom, D
Kammerer, S
Honisch, C
Adam, G
Cantor, CR
Kleyn, P
Braun, A
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (26) : 16871 - 16874
[8] Baron H, 1997, CLIN CHEM, V43, P1984
[9] Barratt BJ, 2002, ANN HUM GENET, V66, P393, DOI [10.1046/j.1469-1809.2002.00125.x, 10.1017/S0003480002001252]
[10] Bell PA, 2002, BIOTECHNIQUES, P70

← 1 2 3 4 5 6 7 8 9 10 →